PUBLICATION

Endotoxin molecule lipopolysaccharide-induced zebrafish inflammation model: a novel screening method for anti-inflammatory drugs

Authors
Yang, L.L., Wang, G.Q., Yang, L.M., Huang, Z.B., Zhang, W.Q., Yu, L.Z.
ID
ZDB-PUB-140513-478
Date
2014
Source
Molecules   19: 2390-409 (Journal)
Registered Authors
Keywords
none
MeSH Terms
  • Interleukin-1beta/metabolism
  • Endotoxins/toxicity
  • Drug Evaluation, Preclinical*
  • Macrophages/drug effects
  • Macrophages/metabolism
  • Neutrophils/drug effects
  • Neutrophils/metabolism
  • Zebrafish*
  • Male
  • Inflammation/chemically induced
  • Inflammation/drug therapy*
  • Inflammation/pathology
  • Chlorogenic Acid/administration & dosage
  • Disease Models, Animal
  • Lipopolysaccharides/toxicity
  • Inflammation Mediators/metabolism
  • Tumor Necrosis Factor-alpha/metabolism
  • Anti-Inflammatory Agents/administration & dosage*
  • Animals
  • Interleukin-6/metabolism
(all 20)
PubMed
24566310 Full text @ Molecules
Abstract

Lipopolysaccharide (LPS), an endotoxin molecule, has been used to induce inflammatory responses. In this study, LPS was used to establish an in vivo inflammation model in zebrafish for drug screening. We present an experimental method that conveniently and rapidly assesses the anti-inflammatory properties of drugs. The yolks of 3-day post-fertilization (dpf) larvae were injected with 0.5 mg/mL LPS to induce fatal inflammation. After LPS stimulation, macrophages were tracked by NR and SB staining and neutrophil migration was observed using the MPO:GFP line. Larval mortality was used as the primary end-point. Expression levels of key cytokines involved in the inflammatory response including IL-1β, IL-6, and TNF-α, were measured using quantitative reverse transcription polymerase chain reaction (RT-PCR). Macrophages and neutrophils were both recruited to the LPS-injected site during the inflammatory response. Mortality was increased by LPS in a dose-dependent manner within 48 h. Analyses of IL-1β, IL-6, and TNF-α expression levels revealed the upregulation of the inflammatory response in the LPS-injected larvae. Further, the anti-inflammatory activity of chlorogenic acid (CA) was evaluated in this zebrafish model to screen for anti-inflammatory drugs. A preliminary result showed that CA revealed a similar effect as the corticosteroid dexamethasone (DEX), which was used as a positive control, by inhibiting macrophage and neutrophil recruitment to the LPS site and improving survival. Our results suggest that this zebrafish screening model could be applied to study inflammation-mediated diseases. Moreover, the Traditional Chinese Medicine CA displays potential anti-inflammatory activity.

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    Citation
    Yang, L.L., Wang, G.Q., Yang, L.M., Huang, Z.B., Zhang, W.Q., Yu, L.Z. (2014) Endotoxin molecule lipopolysaccharide-induced zebrafish inflammation model: a novel screening method for anti-inflammatory drugs. Molecules. 19:2390-409.