PUBLICATION
Functional motifs composed of morphologically homologous neurons repeated in the hindbrain segments
- Authors
- Neki, D., Nakayama, H., Fujii, T., Matsui-Furusho, H., Oda, Y.
- ID
- ZDB-PUB-140513-463
- Date
- 2014
- Source
- The Journal of neuroscience : the official journal of the Society for Neuroscience 34: 3291-302 (Journal)
- Registered Authors
- Oda, Yoichi
- Keywords
- Mauthner cell, escape, hindbrain segments, homologous neurons, reticulospinal neurons, vertebrate
- MeSH Terms
-
- Action Potentials/physiology*
- Animals
- Electric Stimulation
- Female
- Functional Laterality/physiology
- Goldfish
- Male
- Neural Pathways/physiology
- Neurons/classification
- Neurons/physiology*
- Patch-Clamp Techniques
- Reaction Time/physiology*
- Rhombencephalon/cytology*
- Spinal Cord/physiology
- Statistics, Nonparametric
- PubMed
- 24573288 Full text @ J. Neurosci.
Citation
Neki, D., Nakayama, H., Fujii, T., Matsui-Furusho, H., Oda, Y. (2014) Functional motifs composed of morphologically homologous neurons repeated in the hindbrain segments. The Journal of neuroscience : the official journal of the Society for Neuroscience. 34:3291-302.
Abstract
Segmental organization along the neuraxis is a prominent feature of the CNS in vertebrates. In a wide range of fishes, hindbrain segments contain orderly arranged reticulospinal neurons (RSNs). Individual RSNs in goldfish and zebrafish hindbrain are morphologically identified. RSNs sharing similar morphological features are called segmental homologs and repeated in adjacent segments. However, little is known about functional relationships among segmental homologs. Here we investigated the electrophysiological connectivity between the Mauthner cell (M-cell), a pair of giant RSNs in segment 4 (r4) that are known to trigger fast escape behavior, and different series of homologous RSNs in r4-r6. Paired intracellular recordings in adult goldfish revealed unidirectional connections from the M-cell to RSNs. The connectivity was similar in morphological homologs. A single M-cell spike produced IPSPs in dorsally located RSNs (MiD cells) on the ipsilateral side and excitatory postsynaptic depolarization on the contralateral side, except for MiD2cm cells. The inhibitory or excitatory potentials effectively suppressed or enhanced target RSNs spiking, respectively. In contrast to the lateralized effects on MiD cells, single M-cell spiking elicited equally strong depolarizations on bilateral RSNs located ventrally (MiV cells), and the depolarization was high enough for MiV cells to burst. Therefore, the morphological homology of repeated RSNs in r4-r6 and their functional M-cell connectivity were closely correlated, suggesting that each functional connection works as a functional motif during the M-cell-initiated escape.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping