PUBLICATION

Arachidonic acid enhances turnover of the dermal skeleton: studies on zebrafish scales

Authors
de Vrieze, E., Moren, M., Metz, J.R., Flik, G., Lie, K.K.
ID
ZDB-PUB-140513-435
Date
2014
Source
PLoS One   9: e89347 (Journal)
Registered Authors
de Vrieze, Erik, Flik, Gert, Metz, Juriaan R.
Keywords
none
MeSH Terms
  • Animal Structures/drug effects
  • Animal Structures/physiology*
  • Animals
  • Arachidonic Acid/pharmacology*
  • Calcium/metabolism
  • Dermis/drug effects
  • Dermis/physiology*
  • Dinoprostone/metabolism
  • Gene Expression Profiling
  • Matrix Metalloproteinases/metabolism
  • Phosphorus/metabolism
  • Regeneration/drug effects
  • Zebrafish/physiology*
PubMed
24586706 Full text @ PLoS One
Abstract

In fish nutrition, the ratio between omega-3 and omega-6 poly-unsaturated fatty acids influences skeletal development. Supplementation of fish oils with vegetable oils increases the content of omega-6 fatty acids, such as arachidonic acid in the diet. Arachidonic acid is metabolized by cyclooxygenases to prostaglandin E2, an eicosanoid with effects on bone formation and remodeling. To elucidate effects of poly-unsaturated fatty acids on developing and existing skeletal tissues, zebrafish (Danio rerio) were fed (micro-) diets low and high in arachidonic acid content. Elasmoid scales, dermal skeletal plates, are ideal to study skeletal metabolism in zebrafish and were exploited in the present study. The fatty acid profile resulting from a high arachidonic acid diet induced mild but significant increase in matrix resorption in ontogenetic scales of adult zebrafish. Arachidonic acid affected scale regeneration (following removal of ontogenetic scales): mineral deposition was altered and both gene expression and enzymatic matrix metalloproteinase activity changed towards enhanced osteoclastic activity. Arachidonic acid also clearly stimulates matrix metalloproteinase activity in vitro, which implies that resorptive effects of arachidonic acid are mediated by matrix metalloproteinases. The gene expression profile further suggests that arachidonic acid increases maturation rate of the regenerating scale; in other words, enhances turnover. The zebrafish scale is an excellent model to study how and which fatty acids affect skeletal formation.

Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes