PUBLICATION

Multilayer mounting for long-term light sheet microscopy of zebrafish

Authors
Weber, M., Mickoleit, M., Huisken, J.
ID
ZDB-PUB-140513-335
Date
2014
Source
Journal of visualized experiments : JoVE   (84): e51119 (Journal)
Registered Authors
Huisken, Jan, Mickoleit, Michaela
Keywords
none
MeSH Terms
  • Animals
  • Female
  • Male
  • Microscopy/instrumentation*
  • Microscopy/methods*
  • Sepharose
  • Time-Lapse Imaging/instrumentation*
  • Time-Lapse Imaging/methods*
  • Zebrafish/embryology*
PubMed
24637614 Full text @ J. Vis. Exp.
Abstract
Light sheet microscopy is the ideal imaging technique to study zebrafish embryonic development. Due to minimal photo-toxicity and bleaching, it is particularly suited for long-term time-lapse imaging over many hours up to several days. However, an appropriate sample mounting strategy is needed that offers both confinement and normal development of the sample. Multilayer mounting, a new embedding technique using low-concentration agarose in optically clear tubes, now overcomes this limitation and unleashes the full potential of light sheet microscopy for real-time developmental biology.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping