PUBLICATION
Early life perfluorooctanesulphonic acid (PFOS) exposure impairs zebrafish organogenesis
- Authors
- Chen, J., Tanguay, R.L., Tal, T.L., Gai, Z., Ma, X., Bai, C., Tilton, S.C., Jin, D., Yang, D., Huang, C., Dong, Q.
- ID
- ZDB-PUB-140513-277
- Date
- 2014
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 150: 124-32 (Journal)
- Registered Authors
- Keywords
- Developmental toxicity, Gut, Perfluorooctanesulfonic acid, Swim bladder, Zebrafish embryo
- MeSH Terms
-
- Air Sacs/embryology
- Alkanesulfonic Acids/toxicity*
- Animals
- Embryo, Nonmammalian
- Environmental Exposure
- Fluorocarbons/toxicity*
- Gene Expression Regulation, Developmental/drug effects
- Intestines/embryology
- Organogenesis/drug effects*
- Water Pollutants, Chemical/toxicity*
- Zebrafish*/embryology
- Zebrafish*/genetics
- PubMed
- 24667235 Full text @ Aquat. Toxicol.
- CTD
- 24667235
Citation
Chen, J., Tanguay, R.L., Tal, T.L., Gai, Z., Ma, X., Bai, C., Tilton, S.C., Jin, D., Yang, D., Huang, C., Dong, Q. (2014) Early life perfluorooctanesulphonic acid (PFOS) exposure impairs zebrafish organogenesis. Aquatic toxicology (Amsterdam, Netherlands). 150:124-32.
Abstract
As a persistent organic contaminant, perfluorooctanesulphonic acid (PFOS) has been widely detected in the environment, wildlife, and humans. The present study revealed that zebrafish embryos exposed to 16 μM PFOS during a sensitive window of 48-96hour post-fertilization (hpf) disrupted larval morphology at 120hpf. Malformed zebrafish larvae were characterized by uninflated swim bladder, less developed gut, and curved spine. Histological and ultrastructural examination of PFOS-exposed larvae showed structural alterations in swim bladder and gut. Whole genome microarray was used to identify the early transcripts dysregulated following exposure to 16 μM PFOS at 96hpf. In total, 1278 transcripts were significantly misexpressed (p<0.05) and 211 genes were changed at least two-fold upon PFOS exposure in comparison to the vehicle-exposed control group. A PFOS-induced network of perturbed transcripts relating to swim bladder and gut development revealed that misexpression of genes were involved in organogenesis. Taken together, early life stage exposure to PFOS perturbs various molecular pathways potentially resulting in observed defects in swim bladder and gut development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping