PUBLICATION
Identification of critical phosphorylation sites on the carboxy tail of melanopsin
- Authors
- Blasic, J.R., Matos-Cruz, V., Ujla, D., Cameron, E.G., Hattar, S., Halpern, M.E., Robinson, P.R.
- ID
- ZDB-PUB-140513-257
- Date
- 2014
- Source
- Biochemistry 53: 2644-9 (Journal)
- Registered Authors
- Halpern, Marnie E., Matos-Cruz, Vanessa
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- HEK293 Cells
- Humans
- Kinetics
- Light
- Mice
- Molecular Sequence Data
- Multigene Family
- Mutation
- Phosphorylation
- Protein Structure, Tertiary
- Rod Opsins/genetics
- Rod Opsins/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 24678795 Full text @ Biochemistry
Citation
Blasic, J.R., Matos-Cruz, V., Ujla, D., Cameron, E.G., Hattar, S., Halpern, M.E., Robinson, P.R. (2014) Identification of critical phosphorylation sites on the carboxy tail of melanopsin. Biochemistry. 53:2644-9.
Abstract
Light-activated opsins undergo carboxy-terminal phosphorylation, which contributes to the deactivation of their photoresponse. The photopigment melanopsin possesses an unusually long carboxy tail containing 37 serine and threonine sites that are potential sites for phosphorylation by a G-protein dependent kinase (GRK). Here, we show that a small cluster of six to seven sites is sufficient for deactivation of light-activated mouse melanopsin. Surprisingly, these sites are distinct from those that regulate deactivation of rhodopsin. In zebrafish, there are five different melanopsin genes that encode proteins with distinct carboxy-terminal domains. Naturally occurring changes in the same cluster of phosphorylatable amino acids provides diversity in the deactivation kinetics of the zebrafish proteins. These results suggest that variation in phosphorylation sites provides flexibility in the duration and kinetics of melanopsin-mediated light responses.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping