ZFIN ID: ZDB-PUB-140513-227
Excess PLAC8 promotes an unconventional ERK2-dependent EMT in colon cancer
Li, C., Ma, H., Wang, Y., Cao, Z., Graves-Deal, R., Powell, A.E., Starchenko, A., Ayers, G.D., Washington, M.K., Kamath, V., Desai, K., Gerdes, M.J., Solnica-Krezel, L., Coffey, R.J.
Date: 2014
Source: J. Clin. Invest.   124(5): 2172-87 (Journal)
Registered Authors: Ma, Haiting, Solnica-Krezel, Lilianna, Wang, Yang
Keywords: none
MeSH Terms:
  • Animals
  • Cadherins/biosynthesis
  • Cadherins/genetics
  • Cell Line, Tumor
  • Colonic Neoplasms/genetics
  • Colonic Neoplasms/metabolism*
  • Colonic Neoplasms/pathology
  • Dual Specificity Phosphatase 6
  • Epithelial-Mesenchymal Transition*
  • HEK293 Cells
  • Humans
  • Intestinal Mucosa/metabolism*
  • Intestinal Mucosa/pathology
  • Mice
  • Mitogen-Activated Protein Kinase 1/genetics
  • Mitogen-Activated Protein Kinase 1/metabolism*
  • Neoplasm Invasiveness
  • Neoplasm Proteins/genetics
  • Neoplasm Proteins/metabolism*
  • Proteins/genetics
  • Proteins/metabolism*
  • Vimentin/biosynthesis
  • Vimentin/genetics
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 24691442 Full text @ J. Clin. Invest.
The epithelial-to-mesenchymal transition (EMT) transcriptional program is characterized by repression of E-cadherin (CDH1) and induction of N-cadherin (CDH2), and mesenchymal genes like vimentin (VIM). Placenta-specific 8 (PLAC8) has been implicated in colon cancer; however, how PLAC8 contributes to disease is unknown, and endogenous PLAC8 protein has not been studied. We analyzed zebrafish and human tissues and found that endogenous PLAC8 localizes to the apical domain of differentiated intestinal epithelium. Colon cancer cells with elevated PLAC8 levels exhibited EMT features, including increased expression of VIM and zinc finger E-box binding homeobox 1 (ZEB1), aberrant cell motility, and increased invasiveness. In contrast to classical EMT, PLAC8 overexpression reduced cell surface CDH1 and upregulated P-cadherin (CDH3) without affecting CDH2 expression. PLAC8-induced EMT was linked to increased phosphorylated ERK2 (p-ERK2), and ERK2 knockdown restored cell surface CDH1 and suppressed CDH3, VIM, and ZEB1 upregulation. In vitro, PLAC8 directly bound and inactivated the ERK2 phosphatase DUSP6, thereby increasing p-ERK2. In a murine xenograft model, knockdown of endogenous PLAC8 in colon cancer cells resulted in smaller tumors, reduced local invasion, and decreased p-ERK2. Using MultiOmyx, a multiplex immunofluorescence-based methodology, we observed coexpression of cytosolic PLAC8, CDH3, and VIM at the leading edge of a human colorectal tumor, supporting a role for PLAC8 in cancer invasion in vivo.