PUBLICATION
Zinc finger DHHC-type containing 13 regulates fate specification of ectoderm and mesoderm cell lineages by modulating Smad6 activity
- Authors
- Chen, X., Shi, W., Wang, F., Du, Z., Yang, Y., Gao, M., Yao, Y., He, K., Wang, C., Hao, A.
- ID
- ZDB-PUB-140513-202
- Date
- 2014
- Source
- Stem cells and development 23(16): 1899-909 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Acyltransferases/physiology*
- Animals
- Bone Morphogenetic Proteins/physiology
- Cell Differentiation
- Cell Lineage
- Cells, Cultured
- Ectoderm/cytology*
- Mesoderm/cytology*
- Mice
- Signal Transduction
- Smad6 Protein/metabolism*
- Zebrafish
- PubMed
- 24702307 Full text @ Stem Cells Dev.
Citation
Chen, X., Shi, W., Wang, F., Du, Z., Yang, Y., Gao, M., Yao, Y., He, K., Wang, C., Hao, A. (2014) Zinc finger DHHC-type containing 13 regulates fate specification of ectoderm and mesoderm cell lineages by modulating Smad6 activity. Stem cells and development. 23(16):1899-909.
Abstract
The roles of DHHC-containing proteins in embryonic cell fate specification are not well defined, nor are the underlying mechanisms of their activity well understood. Here, we compared the embryonic function of zinc finger DHHC-type containing (Zdhhc13) in zebrafish embryos and in an in vitro cell model. Zdhhc13, a critical regulator of bone morphogenetic protein (BMP) signaling, specifically bound to Smad6 to induce its perinuclear accumulation and degradation through a mechanism independent of its palmitoyltransferase activity. We showed Zdhhc13 played a crucial role during zebrafish embryogenesis in the control of germ layer specification, particularly in ectoderm and mesoderm differentiation homeostasis. Depletion of Zdhhc13 led to the neuralization of ectoderm and dorsalization of mesoderm in zebrafish embryos. Moreover, Zdhhc13 antagonized Smad6 during BMP-dependent signaling and early lineage decisions in our in vitro cell model. Our results extended the cellular role of Zdhhc13, suggesting that it acts as a regulator in BMP signaling, and established that the embryonic function of Zdhhc13 is in lineage specification.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping