PUBLICATION

Melanocortin receptor accessory protein 2 (MRAP2) interplays with the zebrafish melanocortin 1 receptor (MC1R) but has no effect on its pharmacological profile

Authors
Cortés, R., Agulleiro, M.J., Navarro, S., Guillot, R., Sánchez, E., Cerdá-Reverter, J.M.
ID
ZDB-PUB-140513-185
Date
2014
Source
General and comparative endocrinology   201: 30-6 (Journal)
Registered Authors
Keywords
ACTH, MSH, Pigmentation, Skin, Stress, Zebrafish
MeSH Terms
  • Adrenocorticotropic Hormone/pharmacology
  • Agouti Signaling Protein/genetics
  • Agouti Signaling Protein/metabolism
  • Agouti-Related Protein/genetics
  • Agouti-Related Protein/metabolism
  • Animals
  • Blotting, Western
  • Carrier Proteins/genetics
  • Carrier Proteins/metabolism*
  • Cyclic AMP/metabolism
  • Fasting
  • Fluorescent Antibody Technique
  • Hormones/pharmacology*
  • Immunoenzyme Techniques
  • Immunoprecipitation
  • RNA, Messenger/genetics
  • Real-Time Polymerase Chain Reaction
  • Receptor, Melanocortin, Type 1/genetics
  • Receptor, Melanocortin, Type 1/metabolism*
  • Receptors, G-Protein-Coupled/genetics
  • Receptors, G-Protein-Coupled/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Skin Pigmentation/drug effects
  • Skin Pigmentation/physiology*
  • Stress, Physiological
  • Zebrafish/growth & development
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • alpha-MSH/pharmacology
PubMed
24709359 Full text @ Gen. Comp. Endocrinol.
Abstract
The melanocortin system is probably one of the most complex hormonal systems since it integrates agonist, encoded in the proopiomelanocortin precursor, endogenous antagonist, agouti signaling protein and agouti-related protein, five different G-protein coupled receptors and two accessory proteins. These accessory proteins interact with melanocortin receptors to allow traffic to the plasma membrane or to regulate the pharmacological profile. The MC1R fill the extension locus, which is primarily responsible for the regulation of pigmentation. In zebrafish, both MC1R and MRAP2 system are expressed in the skin. We demonstrate that zebrafish MC1R physically, or closely, interacts with the MRAP2 system, although this interaction did not result in modification of the studied pharmacological profile. However, progressive fasting induced skin darkening but also an upregulation of the MRAP2 expression in the skin, suggesting an unknown role for MRAP2a that could involve receptor desensitization processes. We also demonstrate that crowding stress induces skin darkening and a downregulation of MC1R expression in the skin.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping