PUBLICATION

In Vivo Cell Tracking Using PhOTO Zebrafish

Authors
Dempsey, W.P., Qin, H., Pantazis, P.
ID
ZDB-PUB-140513-172
Date
2014
Source
Methods in molecular biology (Clifton, N.J.)   1148: 217-28 (Chapter)
Registered Authors
Dempsey, William, Pantazis, Periklis (Laki)
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cell Lineage
  • Cell Tracking
  • Female
  • Larva/genetics
  • Luminescent Proteins/biosynthesis
  • Luminescent Proteins/genetics
  • Male
  • Microscopy, Confocal
  • Microscopy, Fluorescence
  • Zebrafish/genetics*
PubMed
24718804 Full text @ Meth. Mol. Biol.
Abstract
By combining the strength of previously described in vivo cell tracking methodologies, we have recently generated a set of transgenic zebrafish lines, called "PhOTO (photoconvertible optical tracking of…)" zebrafish. PhOTO zebrafish lines are suitable for cell tracking during highly dynamic events, including gastrulation, tissue regeneration, tumorigenesis, and cancer/disease progression. Global monitoring of cell shape, cell interactions, e.g., cell intercalations, coordinated division, and cell dynamics are accomplished by using fluorescence imaging of nuclear and plasma membrane fluorescent protein labeling. The irreversible green-to-red photoconversion property of Dendra2 fusions enables noninvasive, specific and high-contrast selection of targeted cells of interest, which greatly simplifies cell tracking and segmentation in time and space. Here we demonstrate photoconversion and in vivo cell tracking using PhOTO zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping