PUBLICATION

Neurotransmitter map of the asymmetric dorsal habenular nuclei of zebrafish

Authors
deCarvalho, T.N., Subedi, A., Rock, J., Harfe, B.D., Thisse, C., Thisse, B., Halpern, M.E., Hong, E.
ID
ZDB-PUB-140513-103
Date
2014
Source
Genesis (New York, N.Y. : 2000)   52(6): 636-55 (Journal)
Registered Authors
deCarvalho, Tagide, Halpern, Marnie E., Hong, Elim, Subedi, Abhi, Thisse, Bernard, Thisse, Christine
Keywords
ano2, epithalamus, gng8, habenula, interpeduncular nucleus, left-right asymmetry, mbnl3, somatostatin
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Gene Expression
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Genes, Reporter
  • Habenula/embryology*
  • Habenula/metabolism
  • Immunohistochemistry
  • Neurons/metabolism
  • Neurotransmitter Agents/genetics
  • Neurotransmitter Agents/metabolism*
  • Organ Specificity/genetics
  • Phenotype
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
PubMed
24753112 Full text @ Genesis
Abstract
The role of the habenular nuclei in modulating fear and reward pathways has sparked a renewed interest in this conserved forebrain region. The bilaterally paired habenular nuclei, each consisting of a medial/dorsal and lateral/ventral nucleus, can be further divided into discrete subdomains whose neuronal populations, precise connectivity, and specific functions are not well understood. An added complexity is that the left and right habenulae show pronounced morphological differences in many non-mammalian species. Notably, the dorsal habenulae of larval zebrafish provide a vertebrate genetic model to probe the development and functional significance of brain asymmetry. Previous reports have described a number of genes that are expressed in the zebrafish habenulae, either in bilaterally symmetric patterns or more extensively on one side of the brain than the other. The goal of our study was to generate a comprehensive map of the zebrafish dorsal habenular nuclei, by delineating the relationship between gene expression domains, comparing the extent of left-right asymmetry at larval and adult stages, and identifying potentially functional subnuclear regions as defined by neurotransmitter phenotype. Although many aspects of habenular organization appear conserved with rodents, the zebrafish habenulae also possess unique properties that may underlie lateralization of their functions.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping