Lens extrusion from Laminin alpha 1 mutant zebrafish
- Authors
- Pathania, M., Semina, E.V., and Duncan, M.K.
- ID
- ZDB-PUB-140502-20
- Date
- 2014
- Source
- TheScientificWorldJournal 2014: 524929 (Journal)
- Registered Authors
- Semina, Elena
- Keywords
- none
- MeSH Terms
-
- Animals
- Basement Membrane/metabolism
- Collagen Type IV/metabolism
- Cornea/metabolism
- Cornea/pathology
- Laminin/genetics*
- Laminin/metabolism
- Lens Capsule, Crystalline/metabolism
- Lens, Crystalline/metabolism*
- Lens, Crystalline/pathology*
- Mutation*
- Phenotype
- Zebrafish/genetics*
- Zebrafish Proteins/genetics*
- PubMed
- 24526906 Full text @ ScientificWorldJournal
We report analysis of the ocular lens phenotype of the recessive, larval lethal zebrafish mutant, lama1a69/a69. Previous work revealed that this mutant has a shortened body axis and eye defects including a defective hyaloid vasculature, focal corneal dysplasia, and loss of the crystalline lens. While these studies highlight the importance of lamininα1 in lens development, a detailed analysis of the lens defects seen in these mutants was not reported. In the present study, we analyze the lenticular anomalies seen in the lama1a69/a69 mutants and show that the lens defects result from the anterior extrusion of lens material from the eye secondary to structural defects in the lens capsule and developing corneal epithelium associated with basement membrane loss. Our analysis provides further insights into the role of the lens capsule and corneal basement membrane in the structural integrity of the developing eye.