PUBLICATION
Blu-ray disk lens as the objective of a miniaturized two-photon fluorescence microscope
- Authors
- Chung, H.Y., Kuo, W.C., Cheng, Y.H., Yu, C.H., Chia, S.H., Lin, C.Y., Chen, J.S., Tsai, H.J., Fedotov, A.B., Ivanov, A.A., Zheltikov, A.M., and Sun, C.K.
- ID
- ZDB-PUB-140429-10
- Date
- 2013
- Source
- Optics express 21(25): 31604-31614 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Compact Disks*
- Equipment Design
- Equipment Failure Analysis
- Image Enhancement/instrumentation*
- Lenses*
- Microscopy, Fluorescence, Multiphoton/instrumentation*
- Miniaturization
- PubMed
- 24514733 Full text @ Opt. Express
Citation
Chung, H.Y., Kuo, W.C., Cheng, Y.H., Yu, C.H., Chia, S.H., Lin, C.Y., Chen, J.S., Tsai, H.J., Fedotov, A.B., Ivanov, A.A., Zheltikov, A.M., and Sun, C.K. (2013) Blu-ray disk lens as the objective of a miniaturized two-photon fluorescence microscope. Optics express. 21(25):31604-31614.
Abstract
In this paper, we examine the performance of a Blu-ray disk (BD) aspheric lens as the objective of a miniaturized scanning nonlinear optical microscope. By combining a single 2D micro-electro mechanical system (MEMS) mirror as the scanner and with different tube lens pairs, the field of view (FOV) of the studied microscope varies from 59 μm × 93 μm up to 178 μm × 280 μm, while the corresponding lateral resolution varies from 0.6 μm to 2 μm for two-photon fluorescence (2PF) signals. With a 34/s video frame rate, in vivo dynamic observation of zebrafish heartbeat through 2PF of the excited green fluorescence protein (GFP) is demonstrated.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping