Cheng, R.K., Jesuthasan, S.J., and Penney, T.B. (2014) Zebrafish forebrain and temporal conditioning. Philosophical transactions of the Royal Society of London. Series B, Biological sciences. 369(1637):20120462.
The rise of zebrafish as a neuroscience research model organism, in conjunction with recent progress in single-cell resolution
whole-brain imaging of larval zebrafish, opens a new window of opportunity for research on interval timing. In this article,
we review zebrafish neuroanatomy and neuromodulatory systems, with particular focus on identifying homologies between the
zebrafish forebrain and the mammalian forebrain. The neuroanatomical and neurochemical basis of interval timing is summarized
with emphasis on the potential of using zebrafish to reveal the neural circuits for interval timing. The behavioural repertoire
of larval zebrafish is reviewed and we demonstrate that larval zebrafish are capable of expecting a stimulus at a precise
time point with minimal training. In conclusion, we propose that interval timing research using zebrafish and whole-brain
calcium imaging at single-cell resolution will contribute to our understanding of how timing and time perception originate
in the vertebrate brain from the level of single cells to circuits.