PUBLICATION

Therapeutic effect of deferoxamine on iron overload-induced inhibition of osteogenesis in a zebrafish model

Authors
Chen, B., Yan, Y.L., Liu, C., Bo, L., Li, G.F., Wang, H., and Xu, Y.J.
ID
ZDB-PUB-140317-28
Date
2014
Source
Calcified tissue international   94(3): 353-360 (Journal)
Registered Authors
Wang, Han, Yan, Yi-Lin
Keywords
none
MeSH Terms
  • Animals
  • Bone and Bones/drug effects*
  • Bone and Bones/metabolism
  • Cell Differentiation/drug effects
  • Deferoxamine/pharmacology*
  • Disease Models, Animal
  • Iron Overload/drug therapy*
  • Osteoblasts/drug effects*
  • Osteoblasts/metabolism
  • Osteogenesis/drug effects*
  • Oxidative Stress/drug effects
  • Reactive Oxygen Species/metabolism
  • Zebrafish
PubMed
24414856 Full text @ Calcif. Tissue Int.
Abstract

Osteoporosis results from an imbalance in bone remodeling, in which osteoclastic bone resorption exceeds osteoblastic bone formation. Iron has recently been recognized as an independent risk factor for osteoporosis. Reportedly, excess iron could promote osteoclast differentiation and bone resorption through the production of reactive oxygen species (ROS). We evaluated the effect of iron on osteoblast differentiation and bone formation in zebrafish and further investigated the potential benefits of deferoxamine (DFO), a powerful iron chelator, in iron-overloaded zebrafish. The zebrafish model of iron overload described in this study demonstrated an apparent inhibition of bone formation, accompanied by decreased expression of osteoblast-specific genes (runx2a, runx2b, osteocalcin, osteopontin, ALP, and collagen type I). The negative effect of iron on osteoblastic activity and bone formation could be attributed to increased ROS generation and oxidative stress. Most importantly, we revealed that DFO was capable of removing whole-body iron and attenuating oxidative stress in iron-overloaded larval zebrafish, which facilitated larval recovery from the reductions in bone formation and osteogenesis induced by iron overload.

Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes