PUBLICATION
Analysis of apoptosis in zebrafish embryos by whole-mount immunofluorescence to detect activated Caspase 3
- Authors
- Sorrells, S., Toruno, C., Stewart, R.A., and Jette, C.
- ID
- ZDB-PUB-140303-6
- Date
- 2013
- Source
- Journal of visualized experiments : JoVE (82): e51060 (Journal)
- Registered Authors
- Jette, Cicely A., Sorrells, Shelly, Stewart, Rodney A., Toruno, Cristhian
- Keywords
- none
- MeSH Terms
-
- Animals
- Apoptosis/physiology*
- Caspase 3/analysis
- Caspase 3/metabolism*
- Embryo Culture Techniques/methods*
- Embryo, Nonmammalian/anatomy & histology
- Embryo, Nonmammalian/cytology
- Enzyme Activation
- Fluorescent Antibody Technique/methods*
- Zebrafish/embryology*
- Zebrafish/metabolism
- PubMed
- 24378359 Full text @ J. Vis. Exp.
Citation
Sorrells, S., Toruno, C., Stewart, R.A., and Jette, C. (2013) Analysis of apoptosis in zebrafish embryos by whole-mount immunofluorescence to detect activated Caspase 3. Journal of visualized experiments : JoVE. (82):e51060.
Abstract
Certain genetic perturbations or exposure to toxins can disrupt normal developmental processes leading to death of specific cell types. The analysis of activated Caspase 3 by whole-mount immunofluorescence in zebrafish embryos reveals stage- and tissue-specific localization of cells specifically undergoing apoptosis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping