PUBLICATION

Zebrafish as a Natural Host Model for Vibrio cholerae Colonization and Transmission

Authors
Runft, D.L., Mitchell, K.C., Abuaita, B.H., Allen, J.P., Bajer, S., Ginsburg, K., Neely, M.N., and Withey, J.H.
ID
ZDB-PUB-140303-2
Date
2014
Source
Applied and environmental microbiology   80(5): 1710-1717 (Journal)
Registered Authors
Neely, Melody N.
Keywords
none
MeSH Terms
  • Animals
  • Disease Models, Animal*
  • Fishes/microbiology
  • Gastrointestinal Tract/microbiology
  • Vibrio cholerae/immunology*
  • Vibrio cholerae/physiology*
  • Zebrafish/microbiology*
PubMed
24375135 Full text @ Appl. Environ. Microbiol.
Abstract

The human diarrheal disease cholera is caused by the aquatic bacterium Vibrio cholerae. V. cholerae in the environment is associated with several varieties of aquatic life, including insect egg masses, shellfish, and vertebrate fish. Here we describe a novel animal model for V. cholerae, the zebrafish. Pandemic V. cholerae strains specifically colonize the zebrafish intestinal tract after exposure in water with no manipulation of the animal required. Colonization occurs in close contact with the intestinal epithelium and mimics colonization observed in mammals. Zebrafish that are colonized by V. cholerae transmit the bacteria to naive fish, which then become colonized. Striking differences in colonization between V. cholerae classical and El Tor biotypes were apparent. The zebrafish natural habitat in Asia heavily overlaps areas where cholera is endemic, suggesting that zebrafish and V. cholerae evolved in close contact with each other. Thus, the zebrafish provides a natural host model for the study of V. cholerae colonization, transmission, and environmental survival.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping