PUBLICATION
Quantitative measurement of blood velocity in zebrafish with optical vector field tomography
- Authors
- Fieramonti, L., Foglia, E.A., Malavasi, S., D'Andrea, C., Valentini, G., Cotelli, F., and Bassi, A.
- ID
- ZDB-PUB-140213-10
- Date
- 2015
- Source
- Journal of biophotonics 8(1-2): 52-9 (Journal)
- Registered Authors
- Cotelli, Franco
- Keywords
- none
- MeSH Terms
-
- Zebrafish/physiology*
- Tomography, Optical/methods*
- Animals
- Blood Circulation*
- Imaging, Three-Dimensional
- PubMed
- 24339189 Full text @ J. Biophotonics
Abstract
Microscopy techniques can readily visualize the finest details of embryo vasculature, but still lack to provide a complete three-dimensional representation of blood flow parameters. We present an in-vivo 3D imaging technique, able to reconstruct the blood cell velocity vector over a large volume of zebrafish embryos. This low cost and relatively simple technique is exploited to quantitatively assess blood velocity in the zebrafish tail at different stages of development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping