Construction and application of site-specific artificial nucleases for targeted gene editing
- Kok, F.O., Gupta, A., Lawson, N.D., and Wolfe, S.A.
- Methods in molecular biology (Clifton, N.J.) 1101: 267-303 (Chapter)
- Registered Authors
- Gupta, Ankit, Lawson, Nathan, Wolfe, Scot A.
- zinc finger nucleases, ZFNs, TALE nucleases, TALENs, zebrafish, modular assembly, golden gate assembly
- MeSH Terms
- Animals, Genetically Modified
- Base Sequence
- DNA Primers/genetics
- Escherichia coli
- Gene Targeting/methods*
- INDEL Mutation*
- Molecular Sequence Data
- Protein Engineering/methods*
- Substrate Specificity
- Zinc Fingers
- 24233786 Full text @ Meth. Mol. Biol.
Kok, F.O., Gupta, A., Lawson, N.D., and Wolfe, S.A. (2014) Construction and application of site-specific artificial nucleases for targeted gene editing. Methods in molecular biology (Clifton, N.J.). 1101:267-303.
Artificial nucleases have developed into powerful tools for introducing precise genome modifications in a wide variety of species. In this chapter the authors provide detailed protocols for rapidly constructing zinc finger nucleases (ZFNs) and TALE nucleases (TALENs) and evaluating their activity for the targeted generation of InDels within the zebrafish genome.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes