ZFIN ID: ZDB-PUB-131202-9
General Method for Regulating Protein Stability with Light
Bonger, K.M., Rakhit, R., Payumo, A.Y., Chen, J.K., and Wandless, T.J.
Date: 2014
Source: ACS Chemical Biology   9(1): 111-5 (Journal)
Registered Authors: Chen, James K.
Keywords: none
MeSH Terms:
  • Animals
  • Avena/chemistry
  • Avena/genetics*
  • Light
  • Luminescent Proteins/chemistry*
  • Luminescent Proteins/genetics*
  • Mice
  • NIH 3T3 Cells
  • Photolysis*
  • Phototropins/chemistry*
  • Phototropins/genetics*
  • Protein Engineering
  • Protein Stability*
  • Protein Structure, Tertiary
  • Recombinant Fusion Proteins/chemistry
  • Recombinant Fusion Proteins/genetics
  • Zebrafish
PubMed: 24180414 Full text @ ACS Chem. Biol.

Post-translational regulation of protein abundance in cells is a powerful tool for studying protein function. Here, we describe a novel genetically encoded protein domain that is degraded upon exposure to nontoxic blue light. We demonstrate that fusion proteins containing this domain are rapidly degraded in cultured cells and in zebrafish upon illumination.