PUBLICATION
Isolation of zebrafish gonads for RNA isolation
- Authors
- Huang, H.Y., and Ketting, R.F.
- ID
- ZDB-PUB-131202-2
- Date
- 2014
- Source
- Methods in molecular biology (Clifton, N.J.) : 183-194 (Chapter)
- Registered Authors
- Ketting, René
- Keywords
- zebrafish, piRNA, germline development, gonads, ovary, testis, RNA, FACS, Vasa
- MeSH Terms
-
- Zebrafish/anatomy & histology
- Zebrafish/embryology*
- Zebrafish/genetics*
- Genotype
- Female
- Male
- Ovary/cytology
- Ovary/metabolism*
- Immunoprecipitation
- Animals
- RNA/isolation & purification*
- Flow Cytometry
- Testis/cytology
- Testis/metabolism*
- Dissection/methods*
- PubMed
- 24178566 Full text @ Meth. Mol. Biol.
Citation
Huang, H.Y., and Ketting, R.F. (2014) Isolation of zebrafish gonads for RNA isolation. Methods in molecular biology (Clifton, N.J.). :183-194.
Abstract
Piwi proteins and piRNAs are abundant in the gonads of various animal species. Gonads from different developmental stages provide us information regarding the function of piRNAs and the PIWI pathway during germline development. Here we describe methods for gonad and germ cell preparation from different developmental stages of zebrafish. We also describe how to use these gonads to purify and characterize piRNAs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping