PUBLICATION

Isolation of zebrafish gonads for RNA isolation

Authors
Huang, H.Y., and Ketting, R.F.
ID
ZDB-PUB-131202-2
Date
2014
Source
Methods in molecular biology (Clifton, N.J.) : 183-194 (Chapter)
Registered Authors
Ketting, René
Keywords
zebrafish, piRNA, germline development, gonads, ovary, testis, RNA, FACS, Vasa
MeSH Terms
  • Animals
  • Dissection/methods*
  • Female
  • Flow Cytometry
  • Genotype
  • Immunoprecipitation
  • Male
  • Ovary/cytology
  • Ovary/metabolism*
  • RNA/isolation & purification*
  • Testis/cytology
  • Testis/metabolism*
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology*
  • Zebrafish/genetics*
PubMed
24178566 Full text @ Meth. Mol. Biol.
Abstract

Piwi proteins and piRNAs are abundant in the gonads of various animal species. Gonads from different developmental stages provide us information regarding the function of piRNAs and the PIWI pathway during germline development. Here we describe methods for gonad and germ cell preparation from different developmental stages of zebrafish. We also describe how to use these gonads to purify and characterize piRNAs.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping