Genomic editing opens new avenues for zebrafish as a model for neurodegeneration
- Authors
- Schmid, B., and Haass, C.
- ID
- ZDB-PUB-131113-11
- Date
- 2013
- Source
- Journal of neurochemistry 127(4): 461-470 (Review)
- Registered Authors
- Haass, Christian, Schmid, Bettina
- Keywords
- CRISPR/Cas (clustered regularity interpsaced short palindromic repeats), genomics, neurodegeneration, TALEN, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Clustered Regularly Interspaced Short Palindromic Repeats
- Deoxyribonucleases/genetics
- Disease Models, Animal*
- Gene Knock-In Techniques
- Gene Knockout Techniques
- Genetic Engineering/methods*
- Genome*
- Humans
- Neurodegenerative Diseases/genetics*
- Zebrafish/genetics*
- Zinc Fingers
- PubMed
- 24117801 Full text @ J. Neurochem.
Zebrafish has become a popular model organism to study human diseases. We will highlight the advantages and limitations of zebrafish as a model organism to study neurodegenerative diseases and introduce zinc finger nucleases, transcription activator-like effector nucleases, and the recently established clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeat-associated system for genome editing. The efficiency of the novel genome editing tools now greatly facilitates knock-out and, importantly, also makes knock-in approaches feasible in zebrafish. Genome editing in zebrafish avoids unspecific phenotypes caused by off-target effects and toxicity as frequently seen in conventional knock-down approaches.