Dynamic and reversible fluorescence imaging of superoxide anion fluctuations in live cells and in vivo
- Authors
- Zhang, W., Li, P., Yang, F., Hu, X., Sun, C., Zhang, W., Chen, D., and Tang, B.
- ID
- ZDB-PUB-131021-17
- Date
- 2013
- Source
- Journal of the American Chemical Society 135(40): 14956-14959 (Journal)
- Registered Authors
- Yang, Fan
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Survival
- Hep G2 Cells
- Hepatocytes/cytology
- Hepatocytes/metabolism
- Humans
- Mice
- Optical Imaging/methods*
- Superoxides/metabolism*
- PubMed
- 24059644 Full text @ J. Am. Chem. Soc.
Overgeneration of reactive oxygen species (ROS) is closely associated with cellular damage and diseases. As superoxide anion (O2▪–) is the precursor of other ROS, exploring O2▪– fluctuations in cells and in vivo is of great significance. To address this critical need, we have developed a novel reversible fluorescent probe with one-photon and two-photon fluorescence properties, which is well suited for monitoring O2▪– fluxes selectively and dynamically. Imaging results substantiate dynamic and reversible fluorescence responses of this probe to intracellular O2▪– under apoptotic stimuli. Moreover, this probe can conveniently visualize changes in O2▪– concentration during reperfusion injury in hepatocytes, zebrafish, and mice, by means of one-photon or two-photon imaging according to depths of various samples. The present study provides a powerful fluorescent imaging tool for dynamic tracking of O2▪– in live cells and in vivo.