header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-PUB-130918-17
Small molecule suppressors of Drosophila Kinesin deficiency rescue motor axon development in a zebrafish model of spinal muscular atrophy
Gassman, A., Hao le, T., Bhoite, L., Bradford, C.L., Chien, C.B., Beattie, C.E, and Manfredi, J.P.
Date: 2013
Source: PLoS One 8(9): e74325 (Journal)
Registered Authors: Beattie, Christine, Chien, Chi-Bin
Keywords: none
MeSH Terms:
  • Amyloid Precursor Protein Secretases/metabolism
  • Amyloid beta-Peptides/biosynthesis
  • Animals
  • Axons/drug effects*
  • Axons/metabolism
  • Disease Models, Animal
  • Drosophila melanogaster/drug effects
  • Drosophila melanogaster/metabolism*
  • Female
  • Indoles/chemistry
  • Indoles/pharmacology*
  • Indoles/therapeutic use
  • Kinesin/deficiency*
  • Larva/drug effects
  • Larva/metabolism
  • Locomotion/drug effects
  • Male
  • Motor Neurons/drug effects*
  • Motor Neurons/metabolism
  • Muscular Atrophy, Spinal/drug therapy
  • Muscular Atrophy, Spinal/pathology*
  • Muscular Atrophy, Spinal/physiopathology
  • Neurites/drug effects
  • Neurites/metabolism
  • Peptide Fragments/biosynthesis
  • Spinal Cord/pathology
  • Zebrafish*
PubMed: 24023935 Full text @ PLoS One
FIGURES
ABSTRACT

Proximal spinal muscular atrophy (SMA) is the most common inherited motor neuropathy and the leading hereditary cause of infant mortality. Currently there is no effective treatment for the disease, reflecting a need for pharmacologic interventions that restore performance of dysfunctional motor neurons or suppress the consequences of their dysfunction. In a series of assays relevant to motor neuron biology, we explored the activities of a collection of tetrahydroindoles that were reported to alter the metabolism of amyloid precursor protein (APP). In Drosophila larvae the compounds suppressed aberrant larval locomotion due to mutations in the Khc and Klc genes, which respectively encode the heavy and light chains of kinesin-1. A representative compound of this class also suppressed the appearance of axonal swellings (alternatively termed axonal spheroids or neuritic beads) in the segmental nerves of the kinesin-deficient Drosophila larvae. Given the importance of kinesin-dependent transport for extension and maintenance of axons and their growth cones, three members of the class were tested for neurotrophic effects on isolated rat spinal motor neurons. Each compound stimulated neurite outgrowth. In addition, consistent with SMA being an axonopathy of motor neurons, the three axonotrophic compounds rescued motor axon development in a zebrafish model of SMA. The results introduce a collection of small molecules as pharmacologic suppressors of SMA-associated phenotypes and nominate specific members of the collection for development as candidate SMA therapeutics. More generally, the results reinforce the perception of SMA as an axonopathy and suggest novel approaches to treating the disease.

ADDITIONAL INFORMATION