PUBLICATION

Effects of Nanosilver Exposure on Cholinesterase Activities, CD41, and CDF/LIF-Like Expression in ZebraFish (Danio rerio) Larvae

Authors
Myrzakhanova, M., Gambardella, C., Falugi, C., Gatti, A.M., Tagliafierro, G., Ramoino, P., Bianchini, P., and Diaspro, A.
ID
ZDB-PUB-130905-13
Date
2013
Source
BioMed Research International   2013: 205183 (Journal)
Registered Authors
Keywords
none
MeSH Terms
  • Animals
  • Cholinesterases/metabolism*
  • Dose-Response Relationship, Drug
  • Enzyme Activation/drug effects
  • Female
  • Larva
  • Leukemia Inhibitory Factor/metabolism*
  • Metal Nanoparticles/administration & dosage
  • Metal Nanoparticles/toxicity*
  • Platelet Membrane Glycoprotein IIb/metabolism*
  • Pregnancy
  • Prenatal Exposure Delayed Effects/chemically induced
  • Prenatal Exposure Delayed Effects/metabolism*
  • Silver/administration & dosage
  • Silver/toxicity*
  • Survival Rate
  • Zebrafish/metabolism*
  • Zebrafish Proteins/metabolism*
PubMed
23991412 Full text @ Biomed Res. Int.
Abstract

Metal nanosolicoparticles are suspected to cause diseases in a number of organisms, including man. In this paper, we report the effects of nanosilver (Ag, 1–20 nm particles) on the early development of the zebrafish, a well-established vertebrate model. Embryos at the midgastrula stage were exposed to concentrations ranging from 100 to 0.001 mg/L to verify the effects on different endpoints: lethality, morphology, expression of cholinergic molecules, and development of the immune system. (1) Relative risk of mortality was exponential in the range between 0.001 and 10 mg/L. Exposure to 100 mg/L caused 100% death of embryos before reaching the tail-bud stage. (2) Developmental anomalies were present in the 72 h larvae obtained from embryos exposed to nanosilver: whole body length, decreased eye dimension, and slow response to solicitation by gentle touch with a needle tip, with a significant threshold at 0.1 mg/L. (3) Dose-dependent inhibition of acetylcholinesterase activity was significant among the exposures, except between 1 mg/L and 10 mg/L. (4) The distribution of CD41+ cells and of CDF/LIF-like immunoreactivity was altered according to the Ag concentration. The possible effect of nanosilver in impairing immune system differentiation through the inhibition of molecules related to the cholinergic system is discussed.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping