Oocyte-Specific H2A Variant H2af1o Is Required for Cell Synchrony Before Mid-Blastula Transition in Early Zebrafish Embryos

Yue, H.M., Li, Z., Wu, N., Liu, Z., Wang, Y., and Gui, J.F.
Biology of reproduction   89(4): 82 (Journal)
Registered Authors
Gui, Jian-Fang, Wang, Yang
cell synchrony, early cleavage, histone variant, mid-blastula transition, oocyte-specific histone
MeSH Terms
  • Animals
  • Blastula/cytology
  • Blastula/drug effects
  • Blastula/metabolism*
  • Cell Division/drug effects
  • Female
  • Gastrulation/drug effects
  • Gene Knockdown Techniques
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Histones/antagonists & inhibitors
  • Histones/chemistry
  • Histones/genetics
  • Histones/metabolism*
  • Morpholinos/pharmacology
  • Nuclear Localization Signals/antagonists & inhibitors
  • Nuclear Localization Signals/chemistry
  • Nuclear Localization Signals/genetics
  • Nuclear Localization Signals/metabolism*
  • Nucleosomes/drug effects
  • Nucleosomes/metabolism
  • Oocytes/cytology
  • Oocytes/drug effects
  • Oocytes/metabolism*
  • Oogenesis*/drug effects
  • Organ Specificity
  • Peptide Fragments/antagonists & inhibitors
  • Peptide Fragments/chemistry
  • Peptide Fragments/genetics
  • Peptide Fragments/metabolism
  • Protein Interaction Domains and Motifs
  • Protein Isoforms/antagonists & inhibitors
  • Protein Isoforms/chemistry
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism
  • Recombinant Fusion Proteins/chemistry
  • Recombinant Fusion Proteins/metabolism
  • Zebrafish/embryology
  • Zebrafish/metabolism*
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
23946537 Full text @ Biol. Reprod.

Oocyte-specific histone variants have been expected to play significant roles in early embryonic development, but the exact evidence and the biological function have remained unclear. Here, we present evidence that H2af1o, an oocyte-specific H2A variant, is required for cell synchrony before mid-blastula transition in early zebrafish embryos. The H2A variant is oocyte-specific, peaks in mature eggs and is supplied to early embryos. We constructed a series of deletion plasmids of the zebrafish h2af1o tagged with EGFP, and determined the main key function regions including nuclear localization signal of N-terminal 25 amino acids and nucleosome binding region of 110-122 amino acid sequence in the C-terminus by microinjecting them into one-cell stage zebrafish embryos. In comparison with ubiquitous H2A.X, the H2af1o was revealed to confer a more open structure than canonical H2A in the nucleosomes. Furthermore, we conducted the h2af1o-specific morpholino knockdown analysis in early embryos of zebrafish, and revealed its biological function for maintaining cell synchrony division because the H2af1o deficiency disturbed cell synchrony in early cleavages before mid-blastula transition. Therefore, our current findings provided the first case to understand the biological function of maternal oocyte-specific histone variants in vertebrates.

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