Epidermal keratinocyte polarity and motility require Ca2+ influx through TRPV1
- Authors
- Graham, D.M., Huang, L., Robinson, K.R., and Messerli, M.A.
- ID
- ZDB-PUB-130903-42
- Date
- 2013
- Source
- Journal of Cell Science 126(Pt 20): 4602-13 (Journal)
- Registered Authors
- Keywords
- Ca2+, Polarity, Motility, TRPV1, Skin
- MeSH Terms
-
- Animals
- Calcium/metabolism*
- Calcium Signaling/physiology*
- Cell Movement/physiology*
- Cell Polarity/physiology*
- Epidermis/cytology
- Epidermis/metabolism
- Keratinocytes/cytology*
- Keratinocytes/metabolism*
- TRPV Cation Channels/metabolism*
- Zebrafish
- Zebrafish Proteins/metabolism*
- PubMed
- 23943873 Full text @ J. Cell Sci.
Ca2+ has long been known to play an important role in cellular polarity and guidance. We studied the role of Ca2+ signaling during random and directed cell migration to better understand whether Ca2+ directs cell motility from the leading edge and which ion channels are involved in this function using primary zebrafish keratinocytes. Rapid linescan and time lapse imaging of Ca2+i during migration and automated image alignment enabled us to characterize and map the spatiotemporal changes in Ca2+i. We show that asymmetric distributions of lamellipodial Ca2+ sparks are encoded in frequency, not amplitude, and correlate with cellular rotation during migration. Directed migration during galvanotaxis increases the frequency of Ca2+ sparks over the entire lamellipod; however, these events do not give rise to asymmetric Ca2+i signals that correlate with turning. We demonstrate that Ca2+ permeable channels within these cells are mechanically activated and include several transient receptor potential family members, including TRPV1. Lastly, we demonstrate that cell motility and Ca2+i activity are affected by TRPV1 pharmacological agents, indicating a novel role for this channel during cell migration.