PUBLICATION
Evaluating the developmental toxicity of trypanocidal nitroaromatic compounds on zebrafish
- Authors
- Buchanan-Kilbey, G., Djumpah, J., Papadopoulou, M.V., Bloomer, W., Hu, L., Wilkinson, S.R., and Ashworth, R.
- ID
- ZDB-PUB-130830-1
- Date
- 2013
- Source
- Acta tropica 128(3): 701-5 (Journal)
- Registered Authors
- Ashworth, Rachel
- Keywords
- toxicity, trypanosome, protozoa, zebrafish, development
- MeSH Terms
-
- Animals
- Drug Evaluation, Preclinical/methods*
- Embryo, Nonmammalian/drug effects*
- High-Throughput Screening Assays/methods
- Nifurtimox/adverse effects
- Nitroimidazoles/adverse effects
- Trypanocidal Agents/adverse effects*
- Zebrafish/embryology*
- PubMed
- 23916507 Full text @ Acta. Trop.
Citation
Buchanan-Kilbey, G., Djumpah, J., Papadopoulou, M.V., Bloomer, W., Hu, L., Wilkinson, S.R., and Ashworth, R. (2013) Evaluating the developmental toxicity of trypanocidal nitroaromatic compounds on zebrafish. Acta tropica. 128(3):701-5.
Abstract
Current therapies against African and American trypanosomiasis are problematic and with no immediate prospect of a vaccine there is an urgent need for cheap, more effective treatments. To aid the drug discovery pipeline, we report a novel in vivo screening approach using zebrafish (Danio rerio) embryos as a means of rapidly assessing a compounds developmental toxicity. This technique, amenable to high-throughput screening, was validated using several trypanocidal nitroaromatic prodrugs including nifurtimox and benznidazole.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping