Transcription alterations of microRNAs, cytochrome P4501A and 3A, and AhR and PXR in the liver of zebrafish exposed to crude microcystins
- Authors
- Li, X., Ma, J., Fang, Q., and Li, Y.
- ID
- ZDB-PUB-130722-21
- Date
- 2013
- Source
- Toxicon : official journal of the International Society on Toxinology 73: 17-22 (Journal)
- Registered Authors
- Keywords
- MicroRNA, cytochrome P450, zebrafish, microcystins, transcription
- MeSH Terms
-
- Zebrafish
- Receptors, Aryl Hydrocarbon/metabolism
- Microcystins/toxicity*
- Aryl Hydrocarbon Hydroxylases/metabolism
- Real-Time Polymerase Chain Reaction
- Animals
- Liver/metabolism*
- Zebrafish Proteins/metabolism
- Oxidoreductases, N-Demethylating/metabolism
- DNA Primers/genetics
- MicroRNAs/metabolism
- Gene Expression Regulation/drug effects*
- Cytochrome P-450 CYP1A1/metabolism*
- Receptors, Steroid/metabolism
- PubMed
- 23851223 Full text @ Toxicon.
MicroRNAs are small non-coding regulatory RNAs that not only control diverse cellular processes but also regulate gene expression induced by environmental chemicals. However, little is known about the role of microRNAs in liver response of fish to the exposure of cyanobacterial hepatotoxin microcystins (MCs). In the present study, the transcription levels of 4 miRNAs (dre-miR-21, dre-miR-122, dre-miR-27b, and dre-miR-148), cytochromes P450s CYP1A1 and CYP3A, and their receptors, aryl hydrocarbon receptor (AhR, for CYP1A1) and pregnane X receptor (PXR, for CYP3A), in the liver of zebrafish were evaluated after 24 h of 50, 200, or 800 μg/L of crude MCs exposure by using the quantitative real-time PCR method. The results showed that MCs-exposure elevated the transcription levels of dre-miR-21 and dre-miR-27b while down-regulated the expressions of dre-miR-122 and dre-miR-148. However, CYP1A1 transcription remained unchanged while mRNA levels of AhRR1 and AhR2 were significantly higher than that of control. Furthermore, the expressions of CYP3A65 and its receptor PXR were up-regulated by MCs-exposure at higher concentrations (200, or 800 μg/L of crude MCs). Therefore we suggest that CYP3A65 and PXR may be involved in the metabolization and detoxification of MCs in zebrafish, which may be regulated by dre-miR-27b. This work might be beneficial for the discovery of new potential diagnostic biomarker and drug target for hepatosis caused by MC.