PUBLICATION
A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases
- Authors
- Lim, S., Wang, Y., Yu, X., Huang, Y., Featherstone, M.S., and Sampath, K.
- ID
- ZDB-PUB-130710-114
- Date
- 2013
- Source
- Genome biology 14(7): R69 (Journal)
- Registered Authors
- Featherstone, Mark, Lim, Shi Min, Sampath, Karuna, Wang, Yin
- Keywords
- none
- MeSH Terms
-
- Alleles
- Animals
- Base Sequence
- Chromosome Deletion*
- Crosses, Genetic
- Deoxyribonucleases/metabolism*
- Embryo, Nonmammalian/metabolism
- Female
- Genetic Loci
- Genomics/methods*
- Germ-Line Mutation/genetics
- Green Fluorescent Proteins/metabolism
- Inheritance Patterns/genetics*
- Male
- Molecular Sequence Data
- RNA/genetics
- Zebrafish/embryology
- Zebrafish/genetics*
- Zinc Fingers/genetics
- PubMed
- 23815890 Full text @ Genome Biol.
Citation
Lim, S., Wang, Y., Yu, X., Huang, Y., Featherstone, M.S., and Sampath, K. (2013) A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases. Genome biology. 14(7):R69.
Abstract
Precise and effective genome-editing tools are essential for functional genomics and gene therapy. Targeting nucleases have been successfully used to edit genomes. However, whole-locus or element-specific deletions abolishing transcript expression have not previously been reported. Here, we show heritable targeting of locus-specific deletions in the zebrafish nodal-related genes squint (sqt) and cyclops (cyc). Our strategy of heritable chromosomal editing can be used for disease modeling, analyzing gene clusters, regulatory regions, and determining the functions of non-coding RNAs in genomes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping