Lim, S., Wang, Y., Yu, X., Huang, Y., Featherstone, M.S., and Sampath, K. (2013) A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases. Genome biology. 14(7):R69.
Precise and effective genome-editing tools are essential for functional genomics and
gene therapy. Targeting nucleases have been successfully used to edit genomes. However,
whole-locus or element-specific deletions abolishing transcript expression have not
previously been reported. Here, we show heritable targeting of locus-specific deletions
in the zebrafish nodal-related genes squint (sqt) and cyclops (cyc). Our strategy
of heritable chromosomal editing can be used for disease modeling, analyzing gene
clusters, regulatory regions, and determining the functions of non-coding RNAs in