Sex specific transcriptional responses of the zebrafish (Danio rerio) brain selenoproteome to acute sodium selenite supplementation
- Authors
- Benner, M.J., Settles, M.L., Murdoch, G.K., Hardy, R.W., and Robison, B.D.
- ID
- ZDB-PUB-130709-7
- Date
- 2013
- Source
- Physiological Genomics 45(15): 653-66 (Journal)
- Registered Authors
- Keywords
- selenium, selenoproteins, nutritional genomics, brain, sex
- Datasets
- GEO:GSE44623
- MeSH Terms
-
- Animals
- Brain/metabolism*
- DNA Primers/genetics
- Dietary Supplements
- Female
- Gene Expression Regulation/drug effects*
- Gene Expression Regulation/physiology
- Male
- Protein Array Analysis
- Real-Time Polymerase Chain Reaction
- Selenoproteins/metabolism*
- Sex Factors
- Sodium Selenite/administration & dosage
- Sodium Selenite/pharmacology*
- Zebrafish/metabolism*
- PubMed
- 23737534 Full text @ Physiol. Genomics
The potential benefits of selenium (Se) supplementation are currently under investigation for prevention of certain cancers and treatment of neurological disorders. However, little is known concerning the response of the brain to increased dietary Se under conditions of Se sufficiency, despite the majority of Se supplementation trials occurring in healthy, Se sufficient subjects. We evaluated the transcriptional response of Se-dependent genes, selenoproteins and the genes necessary for their synthesis (the selenoproteome), in the zebrafish (Danio rerio) brain to supplementation with nutritionally relevant levels of dietary Se (sodium selenite) during conditions of assumed Se sufficiency. We first used a microarray approach to analyze the response of the brain selenoproteome to dietary Se supplementation for 14 days, and then assessed the immediacy and time scale transcriptional response of the brain selenoproteome to 1, 7, and 14 days of Se supplementation using quantitative real-time PCR (qRTPCR). The microarray approach did not indicate large-scale influences of Se on the brain transcriptome as a whole or the selenoproteome specifically; only one non-selenoproteome gene (si:ch73-44m9.2) was significantly differentially expressed. Our qRTPCR results, however, indicate that increases of dietary Se cause small, but significant transcriptional changes within the brain selenoproteome, even after only 1 day of supplementation. These responses were dynamic over a short period of supplementation in a manner highly dependent on sex and the duration of Se supplementation. In nutritional intervention studies, it may be necessary to utilize methods such as qRTPCR, which allow larger sample sizes, for detecting subtle transcriptional changes in the brain.