PUBLICATION

Fibronectin mediates mesendodermal cell fate decisions

Authors
Cheng, P., Andersen, P., Hassel, D., Kaynak, B.L., Limphong, P., Juergensen, L., Kwon, C., and Srivastava, D.
ID
ZDB-PUB-130708-15
Date
2013
Source
Development (Cambridge, England)   140(12): 2587-2596 (Journal)
Registered Authors
Hassel, David
Keywords
fibronectin, endoderm, mesoderm, integrin-β1, wnt
MeSH Terms
  • Fibronectins/genetics
  • Fibronectins/metabolism*
  • RNA, Small Interfering/genetics
  • RNA, Small Interfering/metabolism
  • Fetal Proteins/genetics
  • Fetal Proteins/metabolism
  • Integrin beta1/genetics
  • Integrin beta1/metabolism
  • Mesoderm/cytology
  • Mesoderm/metabolism*
  • Cell Differentiation
  • Animals
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • T-Box Domain Proteins/genetics
  • T-Box Domain Proteins/metabolism
  • Coculture Techniques
  • Wnt Signaling Pathway
  • Mice
  • beta Catenin/genetics
  • beta Catenin/metabolism
  • Embryonic Induction
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Endoderm/cytology
  • Endoderm/metabolism*
  • Gene Expression Regulation, Developmental*
  • Extracellular Matrix/genetics
  • Extracellular Matrix/metabolism
  • Zebrafish/embryology
  • Zebrafish/metabolism
  • Embryonic Stem Cells/cytology
  • Embryonic Stem Cells/metabolism
(all 34)
PubMed
23715551 Full text @ Development
Abstract

Non-cell-autonomous signals often play crucial roles in cell fate decisions during animal development. Reciprocal signaling between endoderm and mesoderm is vital for embryonic development, yet the key signals and mechanisms remain unclear. Here, we show that endodermal cells efficiently promote the emergence of mesodermal cells in the neighboring population through signals containing an essential short-range component. The endoderm-mesoderm interaction promoted precardiac mesoderm formation in mouse embryonic stem cells and involved endodermal production of fibronectin. In vivo, fibronectin deficiency resulted in a dramatic reduction of mesoderm accompanied by endodermal expansion in zebrafish embryos. This event was mediated by regulation of Wnt signaling in mesodermal cells through activation of integrin-β1. Our findings highlight the importance of the extracellular matrix in mediating short-range signals and reveal a novel function of endoderm, involving fibronectin and its downstream signaling cascades, in promoting the emergence of mesoderm.

Genes / Markers
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Allele Construct Type Affected Genomic Region
s870TgTransgenic Insertion
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    Sequence Targeting Reagents
    Target Reagent Reagent Type
    fn1aMO1-fn1aMRPHLNO
    fn1bMO1-fn1bMRPHLNO
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    Engineered Foreign Genes
    Marker Marker Type Name
    GFPEFGGFP
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