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ZFIN ID: ZDB-PUB-130703-23
The Small Molecule Genistein Increases Hepcidin Expression in Human Hepatocytes
Zhen, A.W., Nguyen, N.H., Gibert, Y., Motola, S., Buckett, P., Wessling-Resnick, M., Fraenkel, E., and Fraenkel, P.G.
Date: 2013
Source: Hepatology (Baltimore, Md.)   58(4): 1315-1325 (Journal)
Registered Authors: Fraenkel, Paula, Gibert, Yann
Keywords: hemochromatosis, thalassemia, stat3, bone morphogenic protein, interleukin-6
MeSH Terms:
  • Animals
  • Cell Line, Tumor
  • Cells, Cultured
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Embryonic Development/drug effects
  • Genistein/pharmacology*
  • Hepatocytes/drug effects*
  • Hepatocytes/metabolism*
  • Hepcidins/metabolism*
  • Humans
  • In Vitro Techniques
  • Iron/metabolism
  • Models, Animal
  • STAT3 Transcription Factor/metabolism
  • Signal Transduction/drug effects*
  • Signal Transduction/physiology
  • Smad4 Protein/metabolism
  • Zebrafish/embryology
  • Zebrafish/metabolism
PubMed: 23703590 Full text @ Hepatology
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ABSTRACT

Hepcidin, a peptide hormone that decreases intestinal iron absorption and macrophage iron release, is a potential drug target for patients with iron overload syndromes because its levels are inappropriately low in these individuals. Endogenous stimulants of Hepcidin transcription include bone morphogenic protein 6 (BMP) and interleukin-6 (IL-6) via effects on Smad4 or Stat3, respectively. We conducted a small-scale chemical screen in zebrafish embryos to identify small molecules that modulate hepcidin expression. We found that treatment with the isoflavone genistein from 2852 hours post-fertilization in zebrafish embryos enhanced Hepcidin transcript levels as assessed by whole mount in situ hybridization and quantitative realtime RT-PCR. Genistein's stimulatory effect was conserved in human hepatocytes: genistein treatment of HepG2 cells increased both Hepcidin transcript levels and Hepcidin promoter activity. We found that genistein's effect on Hepcidin expression did not depend on estrogen receptor signaling or increased cellular iron uptake, but was impaired by mutation of either the BMP response elements or the Stat3 binding site in the Hepcidin promoter. RNA-sequencing of transcripts from genistein-treated hepatocytes indicated that genistein upregulated 68% of the transcripts that were upregulated by BMP6, however genistein raised the levels of several transcripts involved in Stat3 signaling that were not upregulated by BMP6. Chromatin-immunoprecipitation and ELISA experiments revealed that genistein enhanced Stat3 binding to the Hepcidin promoter and increased phosphorylation of Stat3 in HepG2 cells. CONCLUSION: Genistein is the first small molecule experimental drug that stimulates Hepcidin expression in vivo and in vitro. These experiments demonstrate the feasibility of identifying and characterizing small molecules that increase Hepcidin expression. Genistein and other candidate molecules may subsequently be developed into new therapies for iron overload syndromes.

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