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ZFIN ID: ZDB-PUB-130610-33
A brief protocol for high-resolution whole mount in situ hybridization in zebrafish
Zhang, C.X., and Liu, F.
Date: 2013
Source: Yi chuan = Hereditas   35(4): 522-528 (Journal)
Registered Authors: Zhang, Chunxia
Keywords: none
MeSH Terms:
  • Animals
  • Gene Expression Regulation
  • In Situ Hybridization/instrumentation
  • In Situ Hybridization/methods*
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Time Factors
  • Zebrafish/genetics*
PubMed: 23659943 Full text @ Yi Chuan
ABSTRACT

Whole mount in situ hybridization (WISH) is a popular technique that is used to study temporal-spatial gene expression at mRNA level in model systems. Sensitive and high-resolution WISH is important for every laboratory that uses this technique to study expression pattern of genes in wildtype and perturbed embryos. Based on the commonly used protocols in fish community, our lab optimizes them to get more sensitive and specific results. Here, we briefly summarize the history of this technology using zebrafish as an example, describe a detailed experimental protocol, and provide troubleshooting for imperfect results and their solution.

ADDITIONAL INFORMATION No data available
ERRATA and NOTES
Article is in Chinese.