|ZFIN ID: ZDB-PUB-130610-19|
TALE nuclease engineering and targeted genome modification
Shen, Y., Xiao, A., Huang, P., Wang, W.Y., Zhu, Z.Y., and Zhang, B.
|Source:||Yi chuan = Hereditas 35(4): 395-409 (Journal)|
|Registered Authors:||Huang, Peng, Shen, Yan, Xiao, An, Zhang, Bo|
|PubMed:||23659930 Full text @ Yi Chuan|
Shen, Y., Xiao, A., Huang, P., Wang, W.Y., Zhu, Z.Y., and Zhang, B. (2013) TALE nuclease engineering and targeted genome modification. Yi chuan = Hereditas. 35(4):395-409.
ABSTRACTArtificial designer nucleases targeting specific DNA sequences open up a new field for reverse genetics study. The rapid development of engineered endonucleases (EENs) enables targeted genome modification theoretically in any species. The construction of transcription activator-like effector nucleases (TALENs) is simpler with higher specificity and less toxicity than zinc-finger nucleases (ZFNs). Here, we summarized the recent progresses and prospects of TALEN technology, with an emphasis on its structure, function, and construction strategies, as well as a collection of species and genes that have been successfully modified by TALENs, especially the application in zebrafish.
ADDITIONAL INFORMATION No data available
ERRATA and NOTESArticle is in Chinese.