PUBLICATION

TALE nuclease engineering and targeted genome modification

Authors
Shen, Y., Xiao, A., Huang, P., Wang, W.Y., Zhu, Z.Y., and Zhang, B.
ID
ZDB-PUB-130610-19
Date
2013
Source
Yi chuan = Hereditas   35(4): 395-409 (Journal)
Registered Authors
Huang, Peng, Shen, Yan, Xiao, An, Zhang, Bo
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Endonucleases/chemistry
  • Endonucleases/genetics*
  • Endonucleases/metabolism
  • Gene Targeting/methods*
  • Genome/genetics*
  • Humans
  • Molecular Sequence Data
  • Protein Engineering/methods*
  • Recombinant Fusion Proteins/chemistry
  • Recombinant Fusion Proteins/genetics*
  • Recombinant Fusion Proteins/metabolism
PubMed
23659930 Full text @ Yi Chuan
Abstract
Artificial designer nucleases targeting specific DNA sequences open up a new field for reverse genetics study. The rapid development of engineered endonucleases (EENs) enables targeted genome modification theoretically in any species. The construction of transcription activator-like effector nucleases (TALENs) is simpler with higher specificity and less toxicity than zinc-finger nucleases (ZFNs). Here, we summarized the recent progresses and prospects of TALEN technology, with an emphasis on its structure, function, and construction strategies, as well as a collection of species and genes that have been successfully modified by TALENs, especially the application in zebrafish.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes
Article is in Chinese.