PUBLICATION

Genetic characterization and in vivo image analysis of novel zebrafish Danio rerio pigment mutants

Authors
Hsu, C.C., Pai, W.Y., Lai, C.Y., Lu, M.W., and Her, G.M.
ID
ZDB-PUB-130605-17
Date
2013
Source
Journal of Fish Biology   82(5): 1671-1683 (Journal)
Registered Authors
Her, Guor Muor
Keywords
deep-tissue imaging, GFP, optical clarity, transgenic
MeSH Terms
  • Image Processing, Computer-Assisted
  • Male
  • Reverse Transcriptase Polymerase Chain Reaction
  • Animals, Genetically Modified
  • Mutation
  • Pigments, Biological/genetics*
  • Zebrafish/genetics*
  • Zebrafish/physiology*
  • Animals
(all 9)
PubMed
23639161 Full text @ J. Fish Biol.
Abstract

This study reports the isolation and characterization of a new type of transparent zebrafish Danio rerio mutant called pinky (pk), which has been visually isolated from a spontaneous mutation in a D. rerio colony. The pk larvae possess complex mutations affecting pigmentation because of missing pigment cells or a dramatic reduction in the chromatophore number. The pk displays a totally colourless phenotype and adult body transplant with no other obvious external morphological abnormalities, except for a red retina. The molecular analysis results in several candidate genes, hps1, ap3m2 and rabggta, implicated in the Hermansky–Pudlak syndrome (HPS) genes associated with HPS in pk. To demonstrate its applications of deep-tissue imaging, this study examines green fluorescent protein alone or with other fluorescent proteins to investigate their capability for using multilabelling purposes in live adult pk. In this study, pk is particularly valuable for tissue cell labelling and internal organogenesis studies because of its optical clarity in the adult body.

Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
as3TgTransgenic Insertion
    as200TgTransgenic Insertion
      two13TgTransgenic Insertion
        two19
          Unknown
          two21
            Unknown
            two22
              Unknown
              1 - 6 of 6
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              Human Disease / Model
              Sequence Targeting Reagents
              Fish
              Antibodies
              Orthology
              Engineered Foreign Genes
              Marker Marker Type Name
              EGFPEFGEGFP
              RFPEFGRFP
              1 - 2 of 2
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              Mapping