Genetic characterization and in vivo image analysis of novel zebrafish Danio rerio pigment mutants
- Authors
- Hsu, C.C., Pai, W.Y., Lai, C.Y., Lu, M.W., and Her, G.M.
- ID
- ZDB-PUB-130605-17
- Date
- 2013
- Source
- Journal of Fish Biology 82(5): 1671-1683 (Journal)
- Registered Authors
- Her, Guor Muor
- Keywords
- deep-tissue imaging, GFP, optical clarity, transgenic
- MeSH Terms
-
- Mutation
- Zebrafish/genetics*
- Zebrafish/physiology*
- Animals
- Image Processing, Computer-Assisted
- Pigments, Biological/genetics*
- Animals, Genetically Modified
- Reverse Transcriptase Polymerase Chain Reaction
- Male
- PubMed
- 23639161 Full text @ J. Fish Biol.
This study reports the isolation and characterization of a new type of transparent zebrafish Danio rerio mutant called pinky (pk), which has been visually isolated from a spontaneous mutation in a D. rerio colony. The pk larvae possess complex mutations affecting pigmentation because of missing pigment cells or a dramatic reduction in the chromatophore number. The pk displays a totally colourless phenotype and adult body transplant with no other obvious external morphological abnormalities, except for a red retina. The molecular analysis results in several candidate genes, hps1, ap3m2 and rabggta, implicated in the Hermansky–Pudlak syndrome (HPS) genes associated with HPS in pk. To demonstrate its applications of deep-tissue imaging, this study examines green fluorescent protein alone or with other fluorescent proteins to investigate their capability for using multilabelling purposes in live adult pk. In this study, pk is particularly valuable for tissue cell labelling and internal organogenesis studies because of its optical clarity in the adult body.