PUBLICATION

Sema4d is required for the development of the hindbrain boundary and skeletal muscle in zebrafish

Authors
Yang, J., Zeng, Z., Wei, J., Jiang, L., Ma, Q., Wu, M., Huang, X., Ye, S., Li, Y., Ma, D., and Gao, Q.
ID
ZDB-PUB-130322-37
Date
2013
Source
Biochemical and Biophysical Research Communications   433(2): 213-9 (Journal)
Registered Authors
Keywords
Sema4d, development, Hindbrain, trunk, apoptosis
MeSH Terms
  • Animals
  • Antigens, CD/genetics
  • Antigens, CD/metabolism*
  • Apoptosis/genetics
  • Biomarkers/metabolism
  • Embryo, Nonmammalian
  • Gene Expression Regulation, Developmental*
  • Gene Knockdown Techniques
  • Muscle, Skeletal/embryology*
  • Muscle, Skeletal/pathology
  • Myosins/genetics
  • Myosins/metabolism
  • Rhombencephalon/abnormalities
  • Rhombencephalon/embryology*
  • Rhombencephalon/pathology
  • Semaphorins/genetics
  • Semaphorins/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
23466355 Full text @ Biochem. Biophys. Res. Commun.
Abstract

Semaphorin4d (SEMA4D), also known as CD100, an oligodendrocyte secreted R-Ras GTPase-activating protein (GAP), affecting axonal growth is involved in a range of processes including cell adhesion, motility, angiogenesis, immune responses and tumour progression. However, its actual physiological mechanisms and its role in development remain unclear. This study has focused on the role of sema4d in the development and expression patterns in zebrafish embryos and the effect of its suppression on development using sema4d-specific antisense morpholino-oligonucleotides. In this study the knockdown of sema4d, expressed at all developmental stages, lead to defects in the hindbrain and trunk structure of zebrafish embryos. In addition, these phenotypes appeared to be associated with the abnormal expression of three hindbrain rhombomere boundary markers, wnt1, epha4a and foxb1.2, and two myogenic regulatory factors, myod and myog. Further, a notable increase of cell apoptosis appeared in the sema4d knockdown embryos, while no obvious reduction in cell proliferation was observed. Collectively, these data suggest that sema4d plays an important role in the development of the hindbrain and skeletal muscle.

Genes / Markers
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping