PUBLICATION
TALEN-mediated precise genome modification by homologous recombination in zebrafish
- Authors
- Zu, Y., Tong, X., Wang, Z., Liu, D., Pan, R., Li, Z., Hu, Y., Luo, Z., Huang, P., Wu, Q., Zhu, Z., Zhang, B., and Lin, S.
- ID
- ZDB-PUB-130309-22
- Date
- 2013
- Source
- Nature Methods 10(4): 329-31 (Journal)
- Registered Authors
- Huang, Peng, Hu, Yingying, Lin, Shuo, Luo, Zhou, Tong, Xiangjun, Wang, Zhanxiang, Wu, Qian, Zhang, Bo, Zhu, Zuoyan, Zu, Yao
- Keywords
- none
- MeSH Terms
-
- Animals
- Base Sequence
- Deoxyribonucleases/genetics
- Deoxyribonucleases/metabolism*
- Exons
- Gene Expression Regulation, Developmental
- Gene Expression Regulation, Enzymologic
- Gene Targeting/methods
- Genetic Engineering/methods
- Genomics
- RNA
- Recombination, Genetic/physiology*
- Tyrosine 3-Monooxygenase/genetics
- Tyrosine 3-Monooxygenase/metabolism
- Zebrafish/embryology
- Zebrafish/genetics*
- PubMed
- 23435258 Full text @ Nat. Methods
Citation
Zu, Y., Tong, X., Wang, Z., Liu, D., Pan, R., Li, Z., Hu, Y., Luo, Z., Huang, P., Wu, Q., Zhu, Z., Zhang, B., and Lin, S. (2013) TALEN-mediated precise genome modification by homologous recombination in zebrafish. Nature Methods. 10(4):329-31.
Abstract
We report gene targeting via homologous recombination in zebrafish. We co-injected fertilized eggs with transcription activator–like effector nuclease mRNAs and a donor vector with long homologous arms targeting the tyrosine hydroxylase (th) locus, and we observed effective gene modification that was transmitted through the germ line. We also successfully targeted two additional genes. Homologous recombination in zebrafish with a dsDNA donor expands the utility of this model organism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping