PUBLICATION

TALEN-mediated precise genome modification by homologous recombination in zebrafish

Authors
Zu, Y., Tong, X., Wang, Z., Liu, D., Pan, R., Li, Z., Hu, Y., Luo, Z., Huang, P., Wu, Q., Zhu, Z., Zhang, B., and Lin, S.
ID
ZDB-PUB-130309-22
Date
2013
Source
Nature Methods   10(4): 329-31 (Journal)
Registered Authors
Huang, Peng, Hu, Yingying, Lin, Shuo, Luo, Zhou, Tong, Xiangjun, Wang, Zhanxiang, Wu, Qian, Zhang, Bo, Zhu, Zuoyan, Zu, Yao
Keywords
none
MeSH Terms
  • Animals
  • Base Sequence
  • Deoxyribonucleases/genetics
  • Deoxyribonucleases/metabolism*
  • Exons
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Enzymologic
  • Gene Targeting/methods
  • Genetic Engineering/methods
  • Genomics
  • RNA
  • Recombination, Genetic/physiology*
  • Tyrosine 3-Monooxygenase/genetics
  • Tyrosine 3-Monooxygenase/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics*
PubMed
23435258 Full text @ Nat. Methods
Abstract

We report gene targeting via homologous recombination in zebrafish. We co-injected fertilized eggs with transcription activator–like effector nuclease mRNAs and a donor vector with long homologous arms targeting the tyrosine hydroxylase (th) locus, and we observed effective gene modification that was transmitted through the germ line. We also successfully targeted two additional genes. Homologous recombination in zebrafish with a dsDNA donor expands the utility of this model organism.

Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes