Identification of HIF-1alpha promoter and expression regulation of HIF-1alpha gene by LPS and hypoxia in zebrafish
- Authors
- Liu, S., Zhu, K., Chen, N., Wang, W., and Wang, H.
- ID
- ZDB-PUB-130222-8
- Date
- 2013
- Source
- Fish physiology and biochemistry 39(5): 1153-63 (Journal)
- Registered Authors
- Keywords
- zebrafish, hypoxia-inducible factor-1α, promoter analysis, hypoxia, LPS
- MeSH Terms
-
- Plasmids/genetics
- CpG Islands/genetics
- Computational Biology
- Base Sequence
- Hypoxia/physiopathology
- Hypoxia/veterinary*
- Gene Expression Regulation/drug effects*
- Luciferases
- Sequence Analysis, DNA/veterinary
- Cloning, Molecular
- Sequence Homology
- Hypoxia-Inducible Factor 1, alpha Subunit/genetics*
- Hypoxia-Inducible Factor 1, alpha Subunit/metabolism*
- Fish Diseases/physiopathology*
- Promoter Regions, Genetic/genetics
- DNA Primers/genetics
- Real-Time Polymerase Chain Reaction/veterinary
- Animals
- Species Specificity
- Molecular Sequence Data
- Lipopolysaccharides/pharmacology*
- Analysis of Variance
- Zebrafish/genetics*
- PubMed
- 23392835 Full text @ Fish Physiol. Biochem.
The ubiquitously expressed hypoxia-inducible factor-1α (HIF-1α) acts as a key transcription factor in regulating metabolism, development, cellular survival, proliferation and pathology under hypoxia condition. Compared to mammals, fish are more vulnerable to hypoxia stress and contamination; however, the regulation of HIF-1α in fish remains obscure. In this study, zebrafish HIF-1α promoter was cloned and found to possess a CpG island located at 97 to +403, but the canonical TATA-box was absent. Aligning 240-bp HIF-1α proximal promoter region of zebrafish with other vertebrates showed more than 82 % identity with cyprinid fishes. Further luciferase analysis suggested that the minimal core promoter might locate at 134 to +97, and several putative transcription factor binding sites were found in this region by bioinformatic analysis. Moreover, it was shown that the zebrafish HIF-1α mRNA was significantly activated by 10 μg/mL lipopolysaccharide (LPS) under hypoxia condition and peaked at 8 h after treatment, suggesting LPS- and hypoxia-regulated zebrafish HIF-1α transcriptional activity in a synergistic pattern. This synergistic effect was closely related to the living environment of fish, indicating that this mechanism would be more conducive to fish survival.