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ZFIN ID: ZDB-PUB-130220-2
Comparative evolutionary genomics of medaka and three-spined stickleback fabp2a and fabp2b genes with fabp2 of zebrafish
Parmar, M.B., and Wright, J.M.
Date: 2013
Source: Genome   56(1): 27-37 (Journal)
Registered Authors: Wright, Jonathan M.
Keywords: fabp2, medaka, three-splined stickleback, teleost, whole-genome duplication
MeSH Terms:
  • Animals
  • Base Sequence
  • Evolution, Molecular*
  • Fatty Acid-Binding Proteins/genetics*
  • Fatty Acid-Binding Proteins/metabolism
  • Gene Duplication
  • Genome
  • Genomics
  • Molecular Sequence Data
  • Oryzias/genetics*
  • Phylogeny
  • Smegmamorpha/genetics*
  • Synteny
  • Transcription, Genetic
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed: 23379336 Full text @ Genome
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ABSTRACT

Here we describe the evolutionary relationship of the duplicated intestinal fatty acid binding protein genes fabp2a and fabp2b from medaka and three-spined stickleback by comparing them to the well-studied fabp2 gene from zebrafish. The duplicated fabp2 genes from medaka and three-spined stickleback consist of four exons separated by three introns, which code for a polypeptide of 132 amino acids. Fabp2a and Fabp2b of medaka and three-spined stickleback share highest sequence identity with zebrafish Fabp2. All Fabp2/FABP2 sequences from vertebrates form a distinct clade in a neighbor-joining phylogenetic tree with a robust 100% bootstrap value, which indicates that the medaka and three-spined stickleback fabp2a and fabp2b are orthologs of zebrafish fabp2. The syntenic genes of fabp2a and fabp2b from medaka and three-spined stickleback were shown to be conserved with the syntenic genes of fabp2/FABP2 from zebrafish and human, evidence that the duplicated fabp2 genes from medaka and three-spined stickleback most likely arose from the teleost-specific whole-genome duplication. The tissue-specific distribution of medaka and three-spined stickleback fabp2a and fabp2b transcripts, and zebrafish fabp2 transcripts, assayed by RT-qPCR suggests the acquisition of new function(s) by the medaka fabp2a, and the distinct evolution of fabp2b compared with fabp2a in the medaka and three-spined stickleback genomes.

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