Comparative evolutionary genomics of medaka and three-spined stickleback fabp2a and fabp2b genes with fabp2 of zebrafish
- Authors
- Parmar, M.B., and Wright, J.M.
- ID
- ZDB-PUB-130220-2
- Date
- 2013
- Source
- Genome 56(1): 27-37 (Journal)
- Registered Authors
- Wright, Jonathan M.
- Keywords
- fabp2, medaka, three-splined stickleback, teleost, whole-genome duplication
- MeSH Terms
-
- Animals
- Base Sequence
- Evolution, Molecular*
- Fatty Acid-Binding Proteins/genetics*
- Fatty Acid-Binding Proteins/metabolism
- Gene Duplication
- Genome
- Genomics
- Molecular Sequence Data
- Oryzias/genetics*
- Phylogeny
- Smegmamorpha/genetics*
- Synteny
- Transcription, Genetic
- Zebrafish/genetics*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 23379336 Full text @ Genome
Here we describe the evolutionary relationship of the duplicated intestinal fatty acid binding protein genes fabp2a and fabp2b from medaka and three-spined stickleback by comparing them to the well-studied fabp2 gene from zebrafish. The duplicated fabp2 genes from medaka and three-spined stickleback consist of four exons separated by three introns, which code for a polypeptide of 132 amino acids. Fabp2a and Fabp2b of medaka and three-spined stickleback share highest sequence identity with zebrafish Fabp2. All Fabp2/FABP2 sequences from vertebrates form a distinct clade in a neighbor-joining phylogenetic tree with a robust 100% bootstrap value, which indicates that the medaka and three-spined stickleback fabp2a and fabp2b are orthologs of zebrafish fabp2. The syntenic genes of fabp2a and fabp2b from medaka and three-spined stickleback were shown to be conserved with the syntenic genes of fabp2/FABP2 from zebrafish and human, evidence that the duplicated fabp2 genes from medaka and three-spined stickleback most likely arose from the teleost-specific whole-genome duplication. The tissue-specific distribution of medaka and three-spined stickleback fabp2a and fabp2b transcripts, and zebrafish fabp2 transcripts, assayed by RT-qPCR suggests the acquisition of new function(s) by the medaka fabp2a, and the distinct evolution of fabp2b compared with fabp2a in the medaka and three-spined stickleback genomes.