Matthews, M., and Varga, Z.M. (2012) Anesthesia and euthanasia in zebrafish. ILAR journal. 53(2):192-204.
Because of the relative ease of embryonic manipulation and observation, the ability to produce a great number of genetic mutations,
efficient screening methods, and the continued advance of molecular genetic tools, such as the progress in sequencing and
mapping of the zebrafish genome, the use of zebrafish (Danio rerio) as a biomedical model organism continues to expand. However, studies involving zebrafish husbandry and veterinary care struggle
to keep pace with scientific progress. This article outlines some of the current, acceptable methods for providing anesthesia
and euthanasia and provides some examples of how performance-based approaches can be used to advance the relatively limited
number of anesthetic and euthanizing techniques available for zebrafish.
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