ZFIN ID: ZDB-PUB-130201-9
Calcium Channel Agonists Protect against Neuromuscular Dysfunction in a Genetic Model of TDP-43 Mutation in ALS
Armstrong, G.A., and Drapeau, P.
Date: 2013
Source: The Journal of neuroscience : the official journal of the Society for Neuroscience   33(4): 1741-1752 (Journal)
Registered Authors: Drapeau, Pierre
Keywords: none
MeSH Terms:
  • Amyotrophic Lateral Sclerosis/genetics
  • Amyotrophic Lateral Sclerosis/pathology*
  • Amyotrophic Lateral Sclerosis/physiopathology*
  • Animals
  • Calcium Channel Agonists/pharmacology*
  • DNA-Binding Proteins/genetics
  • Disease Models, Animal
  • Humans
  • Immunohistochemistry
  • Motor Activity/physiology
  • Mutation
  • Neuromuscular Junction/drug effects
  • Neuromuscular Junction/pathology*
  • Patch-Clamp Techniques
  • Zebrafish
PubMed: 23345247 Full text @ J. Neurosci.
ABSTRACT

TAR DNA binding protein (TDP-43, encoded by the TARDBP gene) has recently been shown to be associated with amyotrophic lateral sclerosis (ALS), but the early pathophysiological deficits causing impairment in motor function are unknown. Here we expressed the wild-type human gene (wtTARDBP) or the ALS mutation G348C (mutTARDBP) in zebrafish larvae and characterized their motor (swimming) activity and the structure and function of their neuromuscular junctions (NMJs). Of these groups only mutTARDBP larvae showed impaired swimming and increased motoneuron vulnerability with reduced synaptic fidelity, reduced quantal transmission, and more orphaned presynaptic and postsynaptic structures at the NMJ. Remarkably, all behavioral and cellular features were stabilized by chronic treatment with either of the L-type calcium channel agonists FPL 64176 or Bay K 8644. These results indicate that expression of mutTARDBP results in defective NMJs and that calcium channel agonists could be novel therapeutics for ALS.

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