ZFIN ID: ZDB-PUB-130124-18
An optimized fluorescent probe for visualizing glutamate neurotransmission
Marvin, J.S., Borghuis, B.G., Tian, L., Cichon, J., Harnett, M.T., Akerboom, J., Gordus, A., Renninger, S.L., Chen, T.W., Bargmann, C.I., Orger, M.B., Schreiter, E.R., Demb, J.B., Gan, W.B., Hires, S.A., and Looger, L.L.
Date: 2013
Source: Nature Methods   10(2): 162-170 (Journal)
Registered Authors: Orger, Mike, Renninger, Sabine
Keywords: none
MeSH Terms:
  • Animals
  • Astrocytes/metabolism
  • Biosensing Techniques
  • Caenorhabditis elegans
  • Calcium Signaling/physiology
  • Escherichia coli Proteins*/chemical synthesis
  • Excitatory Postsynaptic Potentials/physiology
  • Fluorescent Dyes*/chemical synthesis
  • Fluorescent Dyes*/metabolism
  • Glutamic Acid/metabolism*
  • Green Fluorescent Proteins*/chemical synthesis
  • Hippocampus/metabolism
  • Mice
  • Motor Cortex/metabolism
  • Neurons/metabolism
  • Photic Stimulation
  • Pyramidal Cells/metabolism
  • Recombinant Fusion Proteins*/chemical synthesis
  • Retina/physiology
  • Signal-To-Noise Ratio
  • Synaptic Transmission/physiology*
  • Zebrafish
PubMed: 23314171 Full text @ Nat. Methods

We describe an intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) with signal-to-noise ratio and kinetics appropriate for in vivo imaging. We engineered iGluSnFR in vitro to maximize its fluorescence change, and we validated its utility for visualizing glutamate release by neurons and astrocytes in increasingly intact neurological systems. In hippocampal culture, iGluSnFR detected single field stimulus–evoked glutamate release events. In pyramidal neurons in acute brain slices, glutamate uncaging at single spines showed that iGluSnFR responds robustly and specifically to glutamate in situ, and responses correlate with voltage changes. In mouse retina, iGluSnFR-expressing neurons showed intact light-evoked excitatory currents, and the sensor revealed tonic glutamate signaling in response to light stimuli. In worms, glutamate signals preceded and predicted postsynaptic calcium transients. In zebrafish, iGluSnFR revealed spatial organization of direction-selective synaptic activity in the optic tectum. Finally, in mouse forelimb motor cortex, iGluSnFR expression in layer V pyramidal neurons revealed task-dependent single-spine activity during running.