Exploring the toxicity of a bismuth-asparagine coordination polymer on the early development of zebrafish embryos
- Authors
- He, N.N., Li, X., Feng, D.F., Wu, M., Chen, R., Chen, T., Chen, D.Y., and Feng, X.Z.
- ID
- ZDB-PUB-121227-9
- Date
- 2013
- Source
- Chemical Research in Toxicology 26(1): 89-95 (Journal)
- Registered Authors
- Li, Xiang
- Keywords
- none
- MeSH Terms
-
- Animals
- Asparagine/chemistry*
- Bismuth/chemistry*
- Early Growth Response Protein 2/genetics
- Early Growth Response Protein 2/metabolism
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism*
- Embryonic Development/drug effects
- Gene Expression Regulation, Developmental/drug effects
- Myosin Light Chains/metabolism
- Nanoparticles/chemistry*
- Nanoparticles/toxicity
- Otx Transcription Factors/metabolism
- Phenotype
- Polymers/chemistry*
- Polymers/toxicity
- Zebrafish/growth & development
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 23260032 Full text @ Chem. Res. Toxicol.
Nanoparticles have been widely used in the field of nanomedicine. The toxicity and biocompatibility of nanoparticles have became a burning question. Here bismuth-asparagine coordination polymers in sphere (BACP-2) were assessed the toxicity and biocompatibility in living organs using zebrafish embryos as a platform. Our study demonstrates that 1-4 cell stage injected BACP-2 leads to smaller head (especially eyes), shorter body length and bigger pericardial edemas. And this effect exhibits significant concentration-dependent manner. The expressions of genes such as krox20, otx2 and cardiac myosin light chain (cmlc2) indicate that the effect of BACP-2 in head and heart is related with changed gene expression pattern. Epiboly delay is observed, and the expression of no tail (ntl) indicates that epiboly delay results from both the BACP-2's effection in cell movement in gastrulation and growth slowly. These results indicate that BACP-2 exhibit the concentration-dependent cytotoxicity and biocompatibility, which may help us to have a better understanding of the nanotoxicity of bismuth derivatives which need rigorous study to evaluate the toxicity before applied in nanomedicine.