ZFIN ID: ZDB-PUB-121220-2
Optogenetic control of transcription in zebrafish
Liu, H., Gomez, G., Lin, S., Lin, S., and Lin, C.
Date: 2012
Source: PLoS One   7(11): e50738 (Journal)
Registered Authors: Gomez, Gustavo, Lin, Shuo
Keywords: none
MeSH Terms:
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors/metabolism
  • Cryptochromes/metabolism
  • Gene Expression Regulation, Fungal/genetics
  • Gene Expression Regulation, Fungal/radiation effects
  • Light
  • Optogenetics/methods*
  • Saccharomyces cerevisiae/genetics
  • Saccharomyces cerevisiae/metabolism
  • Saccharomyces cerevisiae/radiation effects
  • Transcription, Genetic/genetics*
  • Transcription, Genetic/radiation effects*
  • Zebrafish/genetics*
  • Zebrafish/metabolism
PubMed: 23226369 Full text @ PLoS One

Light inducible protein-protein interactions are powerful tools to manipulate biological processes. Genetically encoded light-gated proteins for controlling precise cellular behavior are a new and promising technology, called optogenetics. Here we exploited the blue light-induced transcription system in yeast and zebrafish, based on the blue light dependent interaction between two plant proteins, blue light photoreceptor Cryptochrome 2 (CRY2) and the bHLH transcription factor CIB1 (CRY-interacting bHLH 1). We demonstrate the utility of this system by inducing rapid transcription suppression and activation in zebrafish.