PRSS23 is essential for the Snail-dependent endothelial to mesenchymal transition during valvulogenesis in zebrafish
- Authors
- Chen, I.H., Wang, H.H., Hsieh, Y.S., Huang, W.C., Yeh, H.I., and Chuang, Y.J.
- ID
- ZDB-PUB-121210-1
- Date
- 2013
- Source
- Cardiovascular research 97(3): 443-453 (Journal)
- Registered Authors
- Chuang, Yung-Jen
- Keywords
- PRSS23, snail, endoMT, cardiac valve formation, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cells, Cultured
- Endothelium, Vascular/cytology
- Endothelium, Vascular/drug effects
- Epithelial-Mesenchymal Transition/physiology*
- Gene Expression Regulation/drug effects
- Heart Valves/embryology*
- Heart Valves/physiology
- Humans
- Models, Animal
- Morpholinos/pharmacology
- RNA, Small Interfering/pharmacology
- Serine Proteases/deficiency
- Serine Proteases/genetics
- Serine Proteases/physiology*
- Signal Transduction/physiology
- Transcription Factors/physiology*
- Transforming Growth Factor beta2/pharmacology
- Zebrafish/embryology*
- Zebrafish/physiology
- Zebrafish Proteins/deficiency
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- PubMed
- 23213106 Full text @ Cardiovasc. Res.
Methods and Results We used a transgenic zebrafish line with fluorescently labeled vasculature as a tool to study the function of PRSS23. We first cloned the zebrafish prss23 and confirmed its sequence conservation with other vertebrate orthologs. Expression of prss23 was detected in the ventricle, atrium and AV canal during zebrafish embryonic development. We found that morpholino knockdown of Prss23 inhibited the endothelial to mesenchymal transition (EndoMT) at the AV canal. Moreover, in human aortic endothelial cell-based assays, PRSS23 knockdown by shRNA not only repressed the TGF-β-induced EndoMT but also reduced Snail transcription, suggesting that Snail signaling is downstream of PRSS23 during EndoMT. We further demonstrated that human PRSS23 and Snail could rescue the prss23 morpholino-induced AV canal defect in zebrafish embryos, indicating that the function of PRSS23 in valvulogenesis is evolutionarily conserved.
Conclusion We demonstrated for the first time that the initiation of EndoMT in valvulogenesis depends on PRSS23-Snail signaling and that the functional role of PRSS23 during AV valve formation is evolutionarily conserved.