Anthrax toxin receptor 2a controls mitotic spindle positioning
- Authors
- Castanon, I., Abrami, L., Holtzer, L., Heisenberg, C.P., van der Goot, F.G., and González-Gaitán, M.
- ID
- ZDB-PUB-121206-29
- Date
- 2013
- Source
- Nature cell biology 15(1): 28-39 (Journal)
- Registered Authors
- Heisenberg, Carl-Philipp
- Keywords
- none
- MeSH Terms
-
- Actins/metabolism
- Animals
- Bacterial Proteins/biosynthesis
- Bacterial Proteins/genetics
- Carrier Proteins/metabolism
- Cell Membrane/metabolism
- Cell Polarity
- Cytoskeleton/metabolism
- Embryo, Nonmammalian/cytology
- Gene Knockdown Techniques
- Germ Layers/cytology
- Germ Layers/metabolism
- Luminescent Proteins/biosynthesis
- Luminescent Proteins/genetics
- Microtubule-Associated Proteins/metabolism
- Mitosis
- Monomeric GTP-Binding Proteins/metabolism
- Monomeric GTP-Binding Proteins/physiology
- Morpholinos/genetics
- Neuropeptides/metabolism
- Protein Transport
- Receptors, Peptide/genetics
- Receptors, Peptide/metabolism
- Receptors, Peptide/physiology*
- Recombinant Fusion Proteins/biosynthesis
- Recombinant Fusion Proteins/genetics
- Spindle Apparatus/metabolism*
- Time-Lapse Imaging
- Wnt Signaling Pathway
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish Proteins/physiology*
- rho-Associated Kinases/metabolism
- PubMed
- 23201782 Full text @ Nat. Cell Biol.
Oriented mitosis is essential during tissue morphogenesis. The Wnt/planar cell polarity (Wnt/PCP) pathway orients mitosis in a number of developmental systems, including dorsal epiblast cell divisions along the animal-vegetal (A-V) axis during zebrafish gastrulation. How Wnt signalling orients the mitotic plane is, however, unknown. Here we show that, in dorsal epiblast cells, anthrax toxin receptor 2a (Antxr2a) accumulates in a polarized cortical cap, which is aligned with the embryonic A-V axis and forecasts the division plane. Filamentous actin (F-actin) also forms an A-V polarized cap, which depends on Wnt/PCP and its effectors RhoA and Rock2. Antxr2a is recruited to the cap by interacting with actin. Antxr2a also interacts with RhoA and together they activate the diaphanous-related formin zDia2. Mechanistically, Antxr2a functions as a Wnt-dependent polarized determinant, which, through the action of RhoA and zDia2, exerts torque on the spindle to align it with the A-V axis.