PUBLICATION

Skeletogenic fate of zebrafish cranial and trunk neural crest

Authors
Kague, E., Gallagher, M., Burke, S., Parsons, M., Franz-Odendaal, T., and Fisher, S.
ID
ZDB-PUB-121205-10
Date
2012
Source
PLoS One   7(11): e47394 (Journal)
Registered Authors
Fisher, Shannon, Franz-Odendaal, Tamara, Parsons, Michael
Keywords
none
MeSH Terms
  • Neural Crest/growth & development
  • Neural Crest/physiology*
  • Skull/physiology*
  • Osteogenesis/physiology*
  • Animals, Genetically Modified
  • Peripheral Nervous System/growth & development
  • Peripheral Nervous System/physiology
  • Animals
  • Zebrafish
  • Ganglia/physiology
(all 10)
PubMed
23155370 Full text @ PLoS One
Abstract

The neural crest (NC) is a major contributor to the vertebrate craniofacial skeleton, detailed in model organisms through embryological and genetic approaches, most notably in chick and mouse. Despite many similarities between these rather distant species, there are also distinct differences in the contribution of the NC, particularly to the calvariae of the skull. Lack of information about other vertebrate groups precludes an understanding of the evolutionary significance of these differences. Study of zebrafish craniofacial development has contributed substantially to understanding of cartilage and bone formation in teleosts, but there is currently little information on NC contribution to the zebrafish skeleton. Here, we employ a two?transgene system based on Cre recombinase to genetically label NC in the zebrafish. We demonstrate NC contribution to cells in the cranial ganglia and peripheral nervous system known to be NC?derived, as well as to a subset of myocardial cells. The indelible labeling also enables us to determine NC contribution to late?forming bones, including the calvariae. We confirm suspected NC origin of cartilage and bones of the viscerocranium, including cartilages such as the hyosymplectic and its replacement bones (hymandibula and symplectic) and membranous bones such as the opercle. The cleithrum develops at the border of NC and mesoderm, and as an ancestral component of the pectoral girdle was predicted to be a hybrid bone composed of both NC and mesoderm tissues. However, we find no evidence of a NC contribution to the cleithrum. Similarly, in the vault of the skull, the parietal bones and the caudal portion of the frontal bones show no evidence of NC contribution. We also determine a NC origin for caudal fin lepidotrichia; the presumption is that these are derived from trunk NC, demonstrating that these cells have the ability to form bone during normal vertebrate development.

Genes / Markers
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
zf195TgTransgenic Insertion
    zf259TgTransgenic Insertion
      zf284TgTransgenic Insertion
        zf285TgTransgenic Insertion
          zf384TgTransgenic Insertion
            1 - 5 of 5
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            No data available
            Fish
            Antibodies
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            CFPEFGCFP
            CreEFGCre
            DsRed2EFGDsRed2
            EGFPEFGEGFP
            mCherryEFGmCherry
            1 - 5 of 5
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            Mapping
            No data available