A Heat-Shock Protein Axis Regulates VEGFR2 Proteolysis, Blood Vessel Development and Repair
- Authors
- Bruns, A.F., Yuldasheva, N., Latham, A.M., Bao, L., Pellet-Many, C., Frankel, P., Stephen, S.L., Howell, G.J., Wheatcroft, S.B., Kearney, M.T., Zachary, I.C., and Ponnambalam, S.
- ID
- ZDB-PUB-121127-12
- Date
- 2012
- Source
- PLoS One 7(11): e48539 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- HSP70 Heat-Shock Proteins/metabolism
- Blood Vessels/drug effects
- Blood Vessels/growth & development*
- Blood Vessels/metabolism
- Intracellular Space/drug effects
- Intracellular Space/metabolism
- HSP90 Heat-Shock Proteins/antagonists & inhibitors
- HSP90 Heat-Shock Proteins/metabolism
- Arteries/drug effects
- Arteries/physiology
- Protein Stability/drug effects
- Wound Healing*/drug effects
- Lactams, Macrocyclic/pharmacology
- Proteolysis*/drug effects
- Models, Biological
- Cell Movement/drug effects
- Heat-Shock Proteins/metabolism*
- Mice, Inbred C57BL
- Protein Transport/drug effects
- Zebrafish
- Humans
- Regeneration/drug effects
- Clathrin/metabolism
- Neovascularization, Physiologic*/drug effects
- Signal Transduction*/drug effects
- Benzoquinones/pharmacology
- Endocytosis/drug effects
- Animals
- Human Umbilical Vein Endothelial Cells/cytology
- Human Umbilical Vein Endothelial Cells/drug effects
- Human Umbilical Vein Endothelial Cells/metabolism
- Vascular Endothelial Growth Factor Receptor-2/metabolism*
- Mice
- PubMed
- 23139789 Full text @ PLoS One
Vascular endothelial growth factor A (VEGF-A) binds to the VEGFR2 receptor tyrosine kinase, regulating endothelial function, vascular physiology and angiogenesis. However, the mechanism underlying VEGFR2 turnover and degradation in this response is unclear. Here, we tested a role for heat-shock proteins in regulating the presentation of VEGFR2 to a degradative pathway. Pharmacological inhibition of HSP90 stimulated VEGFR2 degradation in primary endothelial cells and blocked VEGF-A-stimulated intracellular signaling via VEGFR2. HSP90 inhibition stimulated the formation of a VEGFR2-HSP70 complex. Clathrin-mediated VEGFR2 endocytosis is required for this HSP-linked degradative pathway for targeting VEGFR2 to the endosome-lysosome system. HSP90 perturbation selectively inhibited VEGF-A-stimulated human endothelial cell migration in vitro. A mouse femoral artery model showed that HSP90 inhibition also blocked blood vessel repair in vivo consistent with decreased endothelial regeneration. Depletion of either HSP70 or HSP90 caused defects in blood vessel formation in a transgenic zebrafish model. We conclude that perturbation of the HSP70-HSP90 heat-shock protein axis stimulates degradation of endothelial VEGFR2 and modulates VEGF-A-stimulated intracellular signaling, endothelial cell migration, blood vessel development and repair.