PUBLICATION

Parametric functional maps of visual inputs to the tectum

Authors
Nikolaou, N., Lowe, A.S., Walker, A.S., Abbas, F., Hunter, P.R., Thompson, I.D., and Meyer, M.P.
ID
ZDB-PUB-121102-9
Date
2012
Source
Neuron   76(2): 317-324 (Journal)
Registered Authors
Abbas, Fatima, Hunter, Paul, Meyer, Martin, Nikolaou, Nikolas, Walker, Alison
Keywords
none
MeSH Terms
  • Animals, Genetically Modified
  • Axons/physiology
  • Myosin-Light-Chain Kinase/genetics
  • Green Fluorescent Proteins/genetics
  • Larva
  • Retina/cytology
  • Retina/physiology
  • Visual Pathways/physiology*
  • Calmodulin/genetics
  • Peptide Fragments/genetics
  • Visual Fields/physiology*
  • Brain Mapping*
  • Superior Colliculi/cytology
  • Superior Colliculi/physiology*
  • Retinal Ganglion Cells/physiology
  • Zebrafish
  • Animals
  • Calcium/metabolism
(all 18)
PubMed
23083735 Full text @ Neuron
Abstract

How features of the visual scene are encoded in the population activity of retinal ganglion cells (RGCs) targeting specific regions of the brain is not well understood. To address this, we have used a genetically encoded reporter of presynaptic function (SyGCaMP3) to record visually evoked activity in the population of RGC axons innervating the zebrafish tectum. Using unbiased voxel-wise analysis of SyGCaMP3 signals, we identify three subtypes of direction-selective and two subtypes of orientation-selective retinal input. Composite parametric functional maps generated across many larvae show laminar segregation of direction- and orientation-selective responses and unexpected retinotopic biases in the distribution of functional subtypes. These findings provide a systematic description of the form, organization, and dimensionality of visual inputs to the brain and will serve as a platform for understanding emergent properties in tectal circuits associated with visually driven behavior.

Genes / Markers
Figures
Figure Gallery (8 images)
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
No data available
Sequence Targeting Reagents
Fish
1 - 2 of 2
Show
Antibodies
Orthology
Engineered Foreign Genes
Marker Marker Type Name
EGFPEFGEGFP
GAL4EFGGAL4
GCaMPEFGGCaMP
1 - 3 of 3
Show
Mapping